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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

PROX1 Antibody recognizes endogenous levels of total PROX1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro570 of human PROX1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Prospero homeobox protein 1 (PROX1) is a transcription factor known for its roles in organ development and lymphangiogenesis. It plays a critical role in the development of the CNS, lens, retina, liver, pancreas, and heart, and is considered to be the master regulator of the lymphatic system (1,2). PROX1 initiates the differentiation of lymphatic vasculature from the cardinal vein, where it is regulated by Sox18 (3,4). The PROX1 suppressor COUP-TFII represses the Notch pathway in venous endothelium, which prevents arterialization (4). HIF-1α and HIF-1β mediated hypoxia induces PROX1, which suggests a means of promoting lymphangiogenesis. Since the tumor microenvironment is typically hypoxic, regulation of PROX1 by hypoxia may also explain the up-regulation of this transcription factor in some cancers (2). PROX1 promotes colon cancer progression by down-regulating E-cadherin via miR-9, which promotes epithelial-mesenchymal transition (EMT) and metastasis (5). The PROX1 protein can act as a tumor suppressor in cases of hepatocellular carcinoma. PROX1 represses transcription of TWIST1, a transcription factor that promotes metastasis by binding the E-cadherin promoter. The function of PROX1 in other cancers is an area of active research (6).

1.  Shan, S.F. et al. (2008) FEBS Lett 582, 3723-8.

2.  Zhou, B. et al. (2013) FEBS Lett 587, 724-31.

3.  Aranguren, X.L. et al. (2013) J Cell Sci 126, 1164-75.

4.  Kim, H. et al. (2013) PLoS One 8, e52197.

5.  Lu, M.H. et al. (2012) Clin Cancer Res 18, 6416-25.

6.  Chang, T.M. and Hung, W.C. (2012) FEBS Lett 586, 3746-52.

Entrez-Gene Id 5629
Swiss-Prot Acc. Q92786

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Tween is a registered trademark of ICI Americas, Inc.

PROX1 Antibody