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PhosphoSitePlus® Resource

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W IP   IF     H M Mk
REACTIVITY SENSITIVITY MW (kDa) SOURCE
100 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

HRS Antibody recognizes endogenous levels of total HRS protein.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly670 of human HRS protein. Antibodies are purified by protein A and peptide affinity chromatography.

Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) is a ubiquitously expressed, multidomain-containing protein that is tyrosine phosphorylated upon activation of multiple receptor tyrosine kinases (1). HRS contains a proline-rich region, which may mediate interactions with SH3 domain-containing proteins (1). Research studies have also demonstrated that HRS possesses a phosphatidylinositol 3-phosphate-binding FYVE-type zinc finger domain and a coiled-coil domain that target it to membranes of the endosomal compartment (2-4). HRS also possesses a ubiquitin-interacting motif (UIM) that binds ubiquitinated membrane proteins and, in conjunction with Eps15 and STAM proteins of the ESCRT-0 complex, facilitates their sorting through the endosomal compartment for eventual degradation in the lysosome (5-8). Research studies demonstrate that phosphorylation and ubiquitination of HRS play a role in EGFR intracellular trafficking and degradation (9,10).


1.  Komada, M. and Kitamura, N. (1995) Mol Cell Biol 15, 6213-21.

2.  He, J. et al. (2009) Proteins 76, 852-60.

3.  Raiborg, C. et al. (2001) J Cell Sci 114, 2255-63.

4.  Komada, M. et al. (1997) J Biol Chem 272, 20538-44.

5.  Bache, K.G. et al. (2003) J Biol Chem 278, 12513-21.

6.  Raiborg, C. et al. (2002) Nat Cell Biol 4, 394-8.

7.  Hirano, S. et al. (2006) Nat Struct Mol Biol 13, 272-7.

8.  Urbé, S. et al. (2003) J Cell Sci 116, 4169-79.

9.  Stern, K.A. et al. (2007) Mol Cell Biol 27, 888-98.

10.  Tan, X. et al. (2015) EMBO J 34, 475-90.


Entrez-Gene Id 9146
Swiss-Prot Acc. O14964


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Tween is a registered trademark of ICI Americas, Inc.

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HRS Antibody