Upstream / Downstream


Explore pathways related to this product.

Our U.S. Offices Are Closed

Our U.S. offices are closed in observance of Memorial Day. We will reopen on Tuesday, May 31st.

Thank you for your patience.


Find answers on our FAQs page.


PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


We recommend the following alternatives

W IP   IF     H M Mk
100 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

HRS Antibody recognizes endogenous levels of total HRS protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly670 of human HRS protein. Antibodies are purified by protein A and peptide affinity chromatography.

Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) is a ubiquitously expressed, multidomain-containing protein that is tyrosine phosphorylated upon activation of multiple receptor tyrosine kinases (1). HRS contains a proline-rich region, which may mediate interactions with SH3 domain-containing proteins (1). Research studies have also demonstrated that HRS possesses a phosphatidylinositol 3-phosphate-binding FYVE-type zinc finger domain and a coiled-coil domain that target it to membranes of the endosomal compartment (2-4). HRS also possesses a ubiquitin-interacting motif (UIM) that binds ubiquitinated membrane proteins and, in conjunction with Eps15 and STAM proteins of the ESCRT-0 complex, facilitates their sorting through the endosomal compartment for eventual degradation in the lysosome (5-8). Research studies demonstrate that phosphorylation and ubiquitination of HRS play a role in EGFR intracellular trafficking and degradation (9,10).

1.  Komada, M. and Kitamura, N. (1995) Mol Cell Biol 15, 6213-21.

2.  He, J. et al. (2009) Proteins 76, 852-60.

3.  Raiborg, C. et al. (2001) J Cell Sci 114, 2255-63.

4.  Komada, M. et al. (1997) J Biol Chem 272, 20538-44.

5.  Bache, K.G. et al. (2003) J Biol Chem 278, 12513-21.

6.  Raiborg, C. et al. (2002) Nat Cell Biol 4, 394-8.

7.  Hirano, S. et al. (2006) Nat Struct Mol Biol 13, 272-7.

8.  Urbé, S. et al. (2003) J Cell Sci 116, 4169-79.

9.  Stern, K.A. et al. (2007) Mol Cell Biol 27, 888-98.

10.  Tan, X. et al. (2015) EMBO J 34, 475-90.

Entrez-Gene Id 9146
Swiss-Prot Acc. O14964

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Tween is a registered trademark of ICI Americas, Inc.

HRS Antibody