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SENSITIVITY Isotype
Mouse IgG1

Product Usage Information

Application Dilutions
Western Blotting 1:1000
Immunoprecipitation 1:200
Immunofluorescence 1:200

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

MBP Tag (8G1) Mouse mAb detects recombinant proteins fused to maltose binding protein (MBP).


Species Reactivity: All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with an MBP fusion protein.

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.


One commonly used system is the maltose-binding protein (MBP) (1). In this system, the target gene is inserted in the appropriate vector and expressed. Following purification using an amylose column, the MBP-target protein complex can be eluted from the column with maltose and cleaved with a specific protease to yield the purified target protein (2). An antibody to the MBP tag can also be used to isolate or detect expression of the protein.


1.  di Guan, C. et al. (1988) Gene 67, 21-30.

2.  Kellermann, O.K. and Ferenci, T. (1982) Methods Enzymol. 90, 459-463.



For Research Use Only. Not For Use In Diagnostic Procedures.
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