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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

PLCbeta3 Antibody detects endogenous levels of total PLCbeta3 protein. The antibody does not cross-react with PLCbeta1, PLCbeta2 or PLCbeta4.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residues surrounding carboxy-terminal residues of human PLCbeta3. Antibodies are purified by protein A and peptide affinity chromatography.

Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ and PLCε. The PLCβ subfamily includes four members, PLCβ1-4. All four members of the subfamily are activated by α- or β-γ-subunits of the heterotrimeric G-proteins (2,3).

Phosphorylation is one of the key mechanisms that regulates the activity of PLC. Phosphorylation of Ser1105 by PKA or PKC inhibits PLCβ3 activity (4,5). Ser537 of PLCβ3 is phosphorylated by CaMKII, and this phosphorylation may contribute to the basal activity of PLCβ3. PLCγ is activated by both receptor and nonreceptor tyrosine kinases (6).

PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783 and 1245 (7). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (8).

1.  Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509.

2.  Smrcka, A.V. et al. (1991) Science 251, 804-7.

3.  Margolis, B. et al. (1989) Cell 57, 1101-7.

4.  Taylor, S.J. et al. (1991) Nature 350, 516-8.

5.  Kim, H.K. et al. (1991) Cell 65, 435-41.

6.  Yue, C. et al. (1998) J Biol Chem 273, 18023-7.

7.  Wang, Z. et al. (1998) Mol Cell Biol 18, 590-7.

8.  Yue, C. et al. (2000) J Biol Chem 275, 30220-5.

Entrez-Gene Id 5331
Swiss-Prot Acc. Q01970

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

PLCbeta3 Antibody