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REACTIVITY SENSITIVITY MW (kDa) SOURCE
30 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Cyclin D2 Antibody detects endogenous levels of cyclin D2. It does not cross-react with other family members at physiological levels.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide to corresponding to residues near the amino terminus of cyclin D2. Antibodies are purified by protein A and peptide affinity chromatography.

Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4).


1.  Lukas, J. et al. (1996) Mol Cell Biol 16, 6917-25.

2.  Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81.

3.  Sherr, C.J. (1996) Science 274, 1672-7.

4.  Diehl, J.A. et al. (1997) Genes Dev. 11, 957-972.


Entrez-Gene Id 894
Swiss-Prot Acc. P30279


For Research Use Only. Not For Use In Diagnostic Procedures.
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