Upstream / Downstream


Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee



Find answers on our FAQs page.


Visit PhosphoSitePlus®

PTM information and tools available.


We recommend the following alternatives

W IP         H M R
30 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Cyclin D2 Antibody detects endogenous levels of cyclin D2. It does not cross-react with other family members at physiological levels.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide to corresponding to residues near the amino terminus of cyclin D2. Antibodies are purified by protein A and peptide affinity chromatography.

Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4).

1.  Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81.

2.  Sherr, C.J. (1996) Science 274, 1672-7.

3.  Lukas, J. et al. (1996) Mol Cell Biol 16, 6917-25.

4.  Diehl, J.A. et al. (1997) Genes Dev 11, 957-72.

Entrez-Gene Id 894
Swiss-Prot Acc. P30279

Protein Specific References

Kida A et al. (2007) Oncogene 26, 6630–40

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cyclin D2 Antibody