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REACTIVITY SENSITIVITY MW (kDa) SOURCE
90 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

IGF-I Receptor beta Antibody detects endogenous levels of IGF-IR beta. It does not cross-react with insulin receptor.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal residues of human IGF-IR beta. Antibodies are purified by protein A and peptide affinity chromatography.

Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8).


1.  Adams, T.E. et al. (2000) Cell Mol Life Sci 57, 1050-93.

2.  Baserga, R. (2000) Oncogene 19, 5574-81.

3.  Scheidegger, K.J. et al. (2000) J Biol Chem 275, 38921-8.

4.  Hernández-Sánchez, C. et al. (1995) J Biol Chem 270, 29176-81.

5.  Lopaczynski, W. et al. (2000) Biochem Biophys Res Commun 279, 955-60.

6.  Baserga, R. (1999) Exp Cell Res 253, 1-6.

7.  White, M.F. et al. (1985) J Biol Chem 260, 9470-8.

8.  White, M.F. et al. (1988) J Biol Chem 263, 2969-80.


Entrez-Gene Id 3480
Swiss-Prot Acc. P08069


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