Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

To get local purchase information on this product, click here

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

We recommend the following alternatives

W IP         H
REACTIVITY SENSITIVITY MW (kDa) SOURCE
80 NPM-ALK. 200 ALK. Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

ALK Antibody detects endogenous levels of total ALK and NMP-ALK.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues mapping at the carboxy-terminus of human ALK. Antibodies are purified by protein A and peptide affinity chromatography.

Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor for pleiotrophin (PTN), a growth factor involved in embryonic brain development (1-3). In ALK-expressing cells, PTN induces phosphorylation of both ALK and the downstream effectors IRS-1, Shc, PLCγ, and PI3 kinase (1). ALK was originally discovered as a nucleophosmin (NPM)-ALK fusion protein produced by a translocation (4). Investigators have found that the NPM-ALK fusion protein is a constitutively active, oncogenic tyrosine kinase associated with anaplastic lymphoma (4). Research literature suggests that activation of PLCγ by NPM-ALK may be a crucial step for its mitogenic activity and involved in the pathogenesis of anaplastic lymphomas (5).

A distinct ALK oncogenic fusion protein involving ALK and echinoderm microtubule-associated protein like 4 (EML4) has been described in the research literature from a non-small cell lung cancer (NSCLC) cell line, with corresponding fusion transcripts present in some cases of lung adenocarcinoma. The short, amino-terminal region of the microtubule-associated protein EML4 is fused to the kinase domain of ALK (6-8).


1.  Stoica, G.E. et al. (2001) J Biol Chem 276, 16772-9.

2.  Iwahara, T. et al. (1997) Oncogene 14, 439-49.

3.  Morris, S.W. et al. (1997) Oncogene 14, 2175-88.

4.  Morris, S.W. et al. (1994) Science 263, 1281-4.

5.  Bai, R.Y. et al. (1998) Mol Cell Biol 18, 6951-61.

6.  Takeuchi, K. et al. (2008) Clin Cancer Res 14, 6618-24.

7.  Soda, M. et al. (2007) Nature 448, 561-6.

8.  Rikova, K. et al. (2007) Cell 131, 1190-203.


Entrez-Gene Id 238
Swiss-Prot Acc. Q9UM73


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.