Cat. # | Size | Qty. | Price |
---|---|---|---|
3545S | 100 µl |
|
REACTIVITY | H M R Mk |
SENSITIVITY | Endogenous |
MW (kDa) | 95 |
SOURCE | Rabbit |
Product Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
Human, Mouse, Rat, Monkey
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of Nod1 protein. Antibodies were purified by protein A and peptide affinity chromatography.
Nod1/CARD4 is a cytosolic protein structually related to Apaf-1 and plant drug-resistance proteins that has been implicated in apoptosis and inflammatory responses to certain pathogenic bacteria (1-3). It contains an amino-terminal caspase recruitment domain (CARD) that is linked to a central nucleotide-binding domain (NBD; also known as a NOD domain) and is followed by carboxy-terminal leucine-rich repeats (LRR) (1). Like Apaf-1, Nod1 induces apoptosis by a CARD/NBD-dependent activation of caspase-9 (1). The primary function of Nod1 is thought to be as a sensor for certain pathogenic microbes and triggering inflammatory responses including the activation of NF-κB and JNK pathways (4-6). The LRR of Nod1 appears to be involved in recognition of microbial components and the CARD domain induces NF-κB activation in cooperation with the CARD containing kinase, RICK/RIP2/CARDIAK (1,5,6). Mutations in Nod1 have been linked increased susceptibility to asthma (7) and inflammatory bowel disease (8).
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