Upstream / Downstream


Explore pathways related to this product.

Our U.S. Offices Are Closed

Our U.S. offices are closed in observance of Thanksgiving. We will reopen on Monday, November 30th.

Thank you for your patience.


Find answers on our FAQs page.


Visit PhosphoSitePlus®

PTM information and tools available.


We recommend the following alternatives

W IP   IF     H Mk
87 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

HIF-1β/ARNT Antibody detects endogenous levels of total HIF-1β/ARNT protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human HIF-1β/ARNT. Antibodies are purified by protein A and peptide affinity chromatography.

Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism, and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3,4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).

HIF-1β is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8). Together with AhR, HIF-1β plays an important role in xenobiotics metabolism (8). In addition, a chromosomal translocation leading to a TEL-ARNT fusion protein is associated with acute myeloblastic leukemia (9). Studies also found that ARNT/HIF-1β expression levels decrease significantly in pancreatic islets from patients with type 2 diabetes, suggesting that HIF-1β plays an important role in pancreatic β-cell function (10).

1.  Sharp, F.R. and Bernaudin, M. (2004) Nat Rev Neurosci 5, 437-48.

2.  Wang, G.L. et al. (1995) Proc Natl Acad Sci U S A 92, 5510-4.

3.  Jaakkola, P. et al. (2001) Science 292, 468-72.

4.  Maxwell, P.H. et al. (1999) Nature 399, 271-5.

5.  Fukuda, R. et al. (2002) J Biol Chem 277, 38205-11.

6.  Jiang, B.H. et al. (2001) Cell Growth Differ 12, 363-9.

7.  Laughner, E. et al. (2001) Mol Cell Biol 21, 3995-4004.

8.  Walisser, J.A. et al. (2004) Proc Natl Acad Sci U S A 101, 16677-82.

9.  Salomon-Nguyen, F. et al. (2000) Proc Natl Acad Sci U S A 97, 6757-62.

10.  Gunton, J.E. et al. (2005) Cell 122, 337-49.

Entrez-Gene Id 405
Swiss-Prot Acc. P27540

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.

HIF-1β/ARNT Antibody