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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb 3079 40 µl
Western Blotting Immunofluorescence
H 48 Rabbit IgG
Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP® Rabbit mAb 2914 40 µl
Western Blotting Immunofluorescence Flow Cytometry
H M R 35, 40, 48 Rabbit IgG
Aurora A (1F8) Mouse mAb 12100 40 µl
Western Blotting Immunofluorescence Flow Cytometry
H Mk 48 Mouse IgG1
Aurora B/AIM1 Antibody 3094 40 µl
Western Blotting Immunoprecipitation Flow Cytometry
H M R Mk 40 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
Western Blotting
All Horse 

Product Description

The Aurora Antibody Sampler Kit provides an economical means to investigate the G2/M phase of the cell cycle. The kit contains enough primary and secondary antibodies to perform four Western blots with each antibody.


Specificity / Sensitivity

Each antibody in the Aurora Antibody Sampler Kit detects endogenous levels of its respective target protein and does not cross-react with other family members.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide and are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with recombinant human proteins or synthetic peptides.

Aurora kinases belong to a highly conserved family of mitotic serine/threonine kinases with three members identified among mammals: Aurora A, B, and C (1,2). Studies on the temporal expression pattern and subcellular localization of Aurora kinases in mitotic cells suggest an association with mitotic structure. Aurora kinase functional influences span from G2 phase to cytokinesis and may be involved in key cell cycle events such as centrosome duplication, chromosome bi-orientation and segregation, cleavage furrow positioning, and ingression (3). Aurora A is detected at the centrosomes, along mitotic spindle microtubules, and in the cytoplasm of mitotically proliferating cells. Aurora A protein levels are low during G1 and S phases and peak during the G2/M phase of the cell cycle. Phosphorylation of Aurora A at Thr288 in its catalytic domain increases kinase activity. Aurora A is involved in centrosome separation, maturation, and spindle assembly and stability. Expression of Aurora B protein also peaks during the G2/M phase of the cell cycle; Aurora B kinase activity peaks at the transition from metaphase to the end of mitosis. Aurora B associates with chromosomes during prophase prior to relocalizing to the spindle at anaphase. Aurora B regulates chromosome segregation through the control of microtubule-kinetochore attachment and cytokinesis. Expression of both Aurora A and Aurora B during the G2/M phase transition is tightly coordinated with histone H3 phosphorylation (4,5); research investigators have observed overexpression of these kinases in a variety of human cancers (2,4). Aurora C localizes to the centrosome from anaphase to cytokinesis and both mRNA and protein levels peak during G2/M phase. Although typical Aurora C expression is limited to the testis, research studies report overexpression of Aurora C is detected in various cancer cell lines (6).


1.  Warner, S.L. et al. (2003) Mol Cancer Ther 2, 589-95.

2.  Katayama, H. et al. (2003) Cancer Metastasis Rev 22, 451-64.

3.  Andrews, P.D. et al. (2003) Curr Opin Cell Biol 15, 672-83.

4.  Pascreau, G. et al. (2003) Prog Cell Cycle Res 5, 369-74.

5.  Crosio, C. et al. (2002) Mol Cell Biol 22, 874-85.

6.  Kimura, M. et al. (1999) J Biol Chem 274, 7334-40.


Entrez-Gene Id 6790, 9212, 6795
Swiss-Prot Acc. O14965, Q96GD4, Q9UQB9


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.