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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
β-Tubulin (9F3) Rabbit mAb 2128 40 µl
Western Blotting Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Mk Z B 55 Rabbit IgG
COX IV (3E11) Rabbit mAb 4850 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H R Mk Z B Pg 17 Rabbit IgG
NUP98 (C39A3) Rabbit mAb 2598 40 µl
Western Blotting Immunoprecipitation Immunofluorescence
H M R Mk 98 Rabbit IgG
CENP-A Antibody 2186 40 µl
Western Blotting Immunofluorescence
H 17 Rabbit 
Fibrillarin (C13C3) Rabbit mAb 2639 40 µl
Western Blotting Immunofluorescence
H M R Mk 37 Rabbit IgG
LC3B (D11) XP® Rabbit mAb 3868 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R 14, 16 Rabbit IgG
Rab5 (C8B1) Rabbit mAb 3547 40 µl
Western Blotting Immunofluorescence
H M R Mk 25 Rabbit IgG
Calnexin (C5C9) Rabbit mAb 2679 40 µl
Western Blotting Immunohistochemistry Immunofluorescence
H Mk 90 Rabbit 
Histone H3 (D1H2) XP® Rabbit mAb 4499 40 µl
Western Blotting Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Mk 17 Rabbit IgG

Product Description

The Cellular Localization IF Antibody Sampler Kit provides an economical means for identification of cellular organelles by fluorescence immnuocytochemistry (IF-IC). This kit includes enough primary antibody to perform at least twenty IF-IC tests or four Western blots with each antibody.


Specificity / Sensitivity

Each antibody in the Cellular Localization IF Antibody Sampler Kit recognizes only it specific target and does not cross-react with other family members. Each antibody has been validated for IF-IC and stains the organelles indicated above. Expression of these proteins may vary in different cells and tissues. Please see www.cellsignal.com for additional specificity/sensitivity information for individual kit components.


Source / Purification

Rabbit monoclonal and polyclonal antibodies are prepared by immunizing animals with a synthetic peptide corresponding to: the amino terminus of human β-tubulin, the sequence of human GAPDH, the sequence of human calnexin, residues surrounding Lys29 of human COX IV, residues surrounding Gly190 of human Rab5 protein, the carboxy-terminal sequence of human histone H3, residues surrounding Pro671 of human NUP98, residues surrounding Thr298 of human fibrillarin, residues near the amino terminus of LC3B, and human CENP-A protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Knowledge of the subcellular location of a protein may reveal the potential role it plays in a variety of cellular processes. One can confirm the subcellular location of a marker that colocalizes with one of the organelle-specific antibodies in this kit. While these antibodies serve as powerful tools for immunofluorescence, they may also be used as western blot controls for fractionated cell lysates.


Entrez-Gene Id 821, 1058, 1327, 2091, 8350, 81631, 4928, 5868, 203068
Swiss-Prot Acc. P27824, P49450, P13073, P22087, P68431, Q9GZQ8, P52948, P20339, P07437


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
DRAQ5® is a registered trademark of Biostatus Limited.
U.S. Patent No. 5,675,063.