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5143
MYPT1 Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

MYPT1 Antibody Sampler Kit #5143

Citations (3)
Western blot analysis of extracts from HeLa and Jurkat cells, untreated or treated with calyculin A #9902, using Phospho-MYPT1 (Ser507) Antibody (upper) or MYPT1 Antibody #2634 (lower).
Western blot analysis of extracts from HeLa and Jurkat cells, untreated or treated with Calyculin A #9902, using Phospho-MYPT1 (Ser668) Antibody (upper) or total MYPT1 Antibody #2634 (lower).
Western blot analysis of extracts from various cell types using Phospho-MYPT1 (Thr853) Antibody.
Western blot analysis of extracts from 293 cells, untreated or treated with the ROCK inhibitor Y27632 (2 minutes at 10 μM) using Phospho-MYPT1 (Thr696) Antibody or total MYPT1 Antibody #2634. Phospho-MYPT1 (Thr696) Antibody was pre-incubated as indicated with phospho-MYPT1 (Thr696) peptide or nonphospho-MYPT1 (Thr696) peptide.
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Western blot analysis of extracts from various cell lines using MYPT1 (D6C1) Rabbit mAb.
Western blot analysis of extracts from HeLa cells, untreated or treated with Calyculin A #9902, using Phospho-MYPT1 (Ser507) Antibody. The phospho-specificity of the antibody was verified by peptide blocking using no peptide (left), phospho-Ser507 peptide (middle) or phospho-Ser668 peptide (right).
Western blot analysis of extracts from HeLa cells, untreated or treated with Calyculin A #9902, using Phospho-MYPT1 (Ser668) Antibody. The phospho-specificity of the antibody was verified by peptide blocking using no peptide (left), phospho-Ser507 peptide (middle) or phospho-Ser668 peptide (right).
Western blot analysis of extracts from C2C12 cells, untreated or treated with with the ROCK inhibitor Y27632 using Phospho-MYPT1 (Thr853) Antibody (upper) or MYPT1 Antibody #2634 (lower).
Immunoprecipitation of MYPT1 from 293 extracts. Lane 1 is 10% input, lane 2 is MYPT1 (D6C1) Rabbit mAb, and lane 3 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900. Western blot analysis was perfomed using MYPT1 (D6C1) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma (left), hepatocellular carcinoma (middle) or gastric carcinoma (right) using MYPT1 (D6C1) Rabbit mAb (top) or MYPT1 Antibody (bottom). These two antibodies detect unique, non-overlapping epitopes on human MYPT1. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining. 
Immunohistochemical analysis of paraffin-embedded normal human breast using MYPT1 (D6C1) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded KMS-11 cell pellet (left, high-expressing) or SK-MEL-28 cell pellet (right, low-expressing) using MYPT1 (D6C1) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded normal human lymph node using MYPT1 (D6C1) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin lymphoma using MYPT1 (D6C1) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human ovarian clear cell carcinoma using MYPT1 (D6C1) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded normal human prostate using MYPT1 (D6C1) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded normal human spleen using MYPT1 (D6C1) Rabbit mAb.
Confocal immunofluorescent analysis of Hep G2 cells using MYPT1 (D6C1) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
To Purchase # 5143
Cat. # Size Qty. Price
5143T
1 Kit  (5 x 20 microliters)

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-MYPT1 (Ser507) Antibody 3040 20 µl
  • WB
H M R 140 Rabbit 
Phospho-MYPT1 (Ser668) Antibody 3048 20 µl
  • WB
H 140 Rabbit 
Phospho-MYPT1 (Thr853) Antibody 4563 20 µl
  • WB
H M R Hm Mk Dg 140 Rabbit 
Phospho-MYPT1 (Thr696) Antibody 5163 20 µl
  • WB
H M R Mk 140 Rabbit 
MYPT1 (D6C1) Rabbit mAb 8574 20 µl
  • WB
  • IP
  • IHC
  • IF
H Mk Dg 140 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Rab Goat 

Product Description

The MYPT1 Antibody Sampler Kit is an economical way to examine the total protein levels of MYPT1 as well as MYPT1 phosphorylated at Ser507, Ser668, Thr853, and Thr696. The kit includes enough primary and secondary antibodies to perform two Western blot experiments.

Specificity / Sensitivity

Each antibody in the MYPT1 Antibody Sampler Kit detects endogenous levels of its target protein. Activation state antibodies detect only target proteins phosphorylated at indicated residues.

Source / Purification

Phospho-specific polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser507, Ser668, Thr853, and Thr696 of human MYPT1. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MYPT1 portein.

Background

Protein phosphatase 1 (PP1) is a ubiquitous eukaryotic protein serine/threonine phosphatase involved in the regulation of various cell functions. Substrate specificity is determined by the binding of a regulatory subunit to the PP1 catalytic subunit (PP1c). It is estimated that over fifty different regulatory subunits exist (1).
The myosin phosphatase holoenzyme is composed of three subunits: PP1c, a targeting/regulatory subunit (MYPT/myosin-binding subunit of myosin phosphatase), and a 20 kDa subunit of unknown function (M20). MYPT binding to PP1cδ alters the conformation of the catalytic cleft and increases enzyme activity and specificity (2). Two MYPT isoforms that are 61% identical have been described. MYPT1 is widely expressed, while MYPT2 expression appears to be exclusive to heart and brain (3). Related family members include MBS85, MYPT3, and TIMAP (4).
Myosin phosphatase regulates the interaction of actin and myosin in response to signaling through the small GTPase Rho. Rho activity inhibits myosin phosphatase via Rho-associated kinase (ROCK). Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization (5,6).
Phospho-MYPT1 (Ser507) and phospho-MYPT1 (Ser668) antibodies are directed at sites that were identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for modification site discovery. Phosphorylation at these sites was discovered using an Akt substrate antibody. Please visit PhosphoSitePlus®, CST's modification site knowledgebase, at www.phosphosite.org for more information.

Pathways

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Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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