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REACTIVITY SENSITIVITY MW (kDa) Isotype
H Endogenous 160 Rabbit IgG

Western blot analysis of extracts from HUVEC and HMVEC cells using Tie2 (D9D10) Rabbit mAb.

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Product Usage Information

Application Dilutions
Western Blotting 1:1000

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Tie2 (D9D10) Rabbit mAb recognizes endogeneous levels of total Tie2 protein.


Species Reactivity: Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg580 of human Tie2 protein.

Tie2/Tek is a receptor tyrosine kinase (RTK) expressed almost exclusively on endothelial cells. It is critical for vasculogenesis and could be important for maintaining endothelial cell survival and integrity in adult blood vessels as well as tumor angiogenesis (1-3). A family of ligands known as the angiopoietins binds to Tie2. Interestingly, these ligands appear to have opposing actions; Angiopoietin-1 (Ang1) and Angiopoietin-4 (Ang4) stimulate tyrosine phosphorylation of Tie2; Angiopoietin-2 (Ang2) and Angiopoietin-3 (Ang3) can inhibit this phosphorylation (4,5). Downstream signaling components, including Grb2, Grb7, Grb14, SHP-2, the p85 subunit of phosphatidylinositol 3-kinase, and p56/Dok-2 interact with Tie2 in a phosphotyrosine-dependent manner through their SH2 or PTB domains (6,7). Tyr992 is located on the putative activation loop of Tie2 and is a major autophosphorylation site (8).


1.  Ward, N.L. and Dumont, D.J. (2002) Semin. Cell Dev. Biol. 13, 19-27.

2.  Jones, N. and Dumont, D.J. (2000) Cancer Metastasis Rev. 19, 13-17.

3.  Partanen, J. and Dumont, D.J. (1999) Curr. Top. Microbiol. Immunol. 237, 159-172.

4.  Ellis, L. M. et al. (2002) Oncology 16, 31-35.

5.  Koh, G. Y. et al. (2002) Exp. Mol. Med. 34, 1-11.

6.  Jones, N. et al. (1999) J. Biol. Chem. 274, 30896-30905.

7.  Jones, N. et al. (2003) Mol. Cell. Biol. 23, 2658-2668.

8.  Murray, B. W. et al. (2001) Biochem. 40, 10243-10253.


Entrez-Gene Id 7010
Swiss-Prot Acc. Q02763


For Research Use Only. Not For Use In Diagnostic Procedures.
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