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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Hexokinase II (3D7) Mouse mAb recognizes endogenous levels of total hexokinase II protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly332 of human hexokinase II protein.

Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3).

1.  WARBURG, O. (1956) Science 123, 309-14.

2.  Semenza, G.L. (2000) Crit Rev Biochem Mol Biol 35, 71-103.

3.  Smith, T.A. (2000) Br J Biomed Sci 57, 170-8.

Entrez-Gene Id 3099
Swiss-Prot Acc. P52789

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Hexokinase II (3D7) Mouse mAb