Upstream / Downstream

Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Stat5 (3H7) Rabbit mAb 9358 100 µl
H M R 90 Rabbit IgG
Phospho-Stat5 (Tyr694) (D47E7) XP® Rabbit mAb 4322 100 µl
H M 90 Rabbit IgG

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.


Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).


1.  Okutani, Y. et al. (2001) Oncogene 20, 6643-6650.

2.  Demoulin, J.B. et al. (1999) J. Biol. Chem. 274, 25855-25861.

3.  Meinke, A. et al. (1996) Mol. Cell. Biol. 16, 6937-6944.

4.  Gouilleux, F. et al. (1994) EMBO J 13, 4361-9.

5.  Wakao, H. et al. (1994) EMBO J 13, 2182-91.

6.  Dentelli, P. et al. (1999) J. Immunol. 163, 2151-2159.


Entrez-Gene Id 6776, 6777
Swiss-Prot Acc. P42229, P51692


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.