Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Our U.S. Offices Are Closed

Our U.S. offices are closed in observance of Thanksgiving. We will reopen on Monday, December 1st.

Thank you for your patience.

To get local purchase information on this product, click here

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Stat1 (42H3) Rabbit mAb 9175 100 µl
Western Blotting Immunohistochemistry
H Mk 84, 91 Rabbit IgG
Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb 7649 100 µl
Western Blotting Immunoprecipitation Immunofluorescence Flow Cytometry Chromatin Immunoprecipitation
H M R 84, 91 Rabbit IgG

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.


The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.


1.  Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.

2.  Ihle, J.N. et al. (1994) Trends Biochem. Sci. 19, 222-227.

3.  Durbin, J.E. et al. (1996) Cell 84, 443-50.

4.  Meraz, M.A. et al. (1996) Cell 84, 431-42.

5.  Wen, Z. et al. (1995) Cell 82, 241-50.

6.  Frank, D.A. (1999) Mol. Med. 5, 432-456.


Entrez-Gene Id 6772
Swiss-Prot Acc. P42224


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.