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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
PKM2 (D78A4) XP® Rabbit mAb 4053 40 µl
H M R Mk 60 Rabbit IgG
GAPDH (D16H11) XP® Rabbit mAb 5174 40 µl
H M R Mk 37 Rabbit 
Pyruvate Dehydrogenase (C54G1) Rabbit mAb 3205 40 µl
H M R Mk 43 Rabbit IgG
Hexokinase I (C35C4) Rabbit mAb 2024 40 µl
H M 102 Rabbit IgG
Hexokinase II (C64G5) Rabbit mAb 2867 40 µl
H M R Mk 102 Rabbit IgG
LDHA (C4B5) Rabbit mAb 3582 40 µl
H Mk 37 Rabbit IgG
PKM1/2 (C103A3) Rabbit mAb 3190 40 µl
H M R Mk 60 Rabbit IgG
PFKP (D4B2) Rabbit mAb 8164 40 µl
H Mk 80 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
All Goat 

Product Description

The Glycolysis Antibody Sampler Kit provides an economical means to investigate select enzymes involved in glycolysis. The kit contains enough primary antibody to perform four western blot experiments with each primary antibody.


Specificity / Sensitivity

GAPDH (D16H11) XP® Rabbit mAb, Hexokinase I (C35C4) Rabbit mAb, Hexokinase II (C64G5) Rabbit mAb, LDHA (C4B5) Rabbit mAb, PKM2 (D78A4) XP® Rabbit mAb, and PFKP (D4B2) Rabbit mAb all detect endogenous levels of their respective total proteins. Pyruvate Dehydrogenase (C54G1) Rabbit mAb detects endogenous levels of total Pyruvate Dehydrogenase α1 subunit. PKM1/2 (C103A3) Rabbit mAb detects endogenous levels of total PKM (including M1 and M2) protein.


Source / Purification

Monoclonal antibodies were produced by immunizing animals with a synthetic peptide corresponding to the respective sequences of human GAPDH, Hexokinase I, Hexokinase II, LDHA, PKM2, Pyruvate Dehydrogenase or PFKP protein.

Glycolysis is the metabolic process by which glucose is converted to pyruvate in a sequence of enzymatic steps. Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg effect) (1). Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate. Platelet-type phosphofructokinase (PFKP) is expressed in various cell types (2). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate (3). Pyruvate kinase, a glycolytic enzyme, catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues. The M2 isoform (PKM2), an alternatively-spliced variant of M1, is expressed during embryonic development (4). Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+ (5). LDHA expression is induced when the oxygen supply is too low for mitochondrial ATP production (6). The pyruvate dehydrogenase complex catalyzes the conversion of pyruvate and CoA into acetyl-CoA and CO2 in the presence of NAD+. The reaction of oxidative decarboxylation of pyruvate serves as a critical link between glycolysis and the citric acid cycle and lipid metabolism (7).


1.  WARBURG, O. (1956) Science 123, 309-14.

2.  Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95.

3.  Christofk, H.R. et al. (2008) Nature 452, 230-3.

4.  Morrison, N. et al. (1992) Hum Genet 89, 105-6.

5.  Semenza, G.L. et al. (1996) J Biol Chem 271, 32529-37.

6.  Strumiło, S. (2005) Acta Biochim Pol 52, 759-64.

7.  Semenza, G.L. (2007) Biochem J 405, 1-9.


Entrez-Gene Id 2597, 3098, 3099, 3939, 5160, 5214, 5315
Swiss-Prot Acc. P04406, P19367, P52789, P00338, P08559, Q01813, P14618


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.