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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
CDT1 (D10F11) Rabbit mAb 8064 40 µl
Western Blotting Immunoprecipitation Immunofluorescence
H Mk 65 Rabbit IgG
MCM2 (D7G11) XP® Rabbit mAb 3619 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Chromatin Immunoprecipitation
H M R Mk 125 Rabbit IgG
MCM3 (D47B6) Rabbit mAb 4003 40 µl
Western Blotting
H M R Mk 100 Rabbit IgG
MCM7 (D10A11) XP® Rabbit mAb 3735 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence
H M R Hm Mk Dg 80 Rabbit IgG
PCNA (PC10) Mouse mAb 2586 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Mk B Pg 36 Mouse IgG2a
RPA70/RPA1 (C24F2) Rabbit mAb 2193 40 µl
Western Blotting
H Mk 70 Rabbit IgG
p58 Primase (8D3) Rat mAb 4726 40 µl
Western Blotting Immunoprecipitation
H M R Hm Mk 58 Rat IgG2a
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
Western Blotting
All Horse 
Anti-rat IgG, HRP-linked Antibody 7077 100 µl
Western Blotting
All Goat 

Product Description

The DNA Replication Antibody Sampler Kit provides a fast and economical means of evaluating multiple targets regulating DNA replication. The kit contains enough primary antibodies to perform four western blots with each antibody.

Specificity / Sensitivity

All antibodies recognize endogenous levels of the respective target protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with recombinant human proteins or synthetic peptides.

The initiation of DNA replication in mammalian cells is a highly coordinated process that is regulated by several protein complexes. Origins of replication (ORCs), at which replication is initiated, are dispersed throughout the genome. Their activities are regulated via the sequential binding of pre-replication and replication factors that initiate formation of replication forks, the active structures at which DNA is synthesized. The origin recognition complex is thought to be bound to chromatin throughout the cell cycle (1,2). The pre-replication complex (Pre-RC) forms in late mitosis/early G1 phase beginning with the binding of CDT1 and CDC6 to the origin. Together CDT1 and CDC6 promote the loading of the heterohexameric minichromosome maintenance (MCM) complex. This process is referred to as chromatin licensing. Licensing of the chromatin permits the DNA to replicate only once per cell cycle, helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). The canonical MCM complex proteins (MCM2-7) are a family of six related phospho-proteins that function, in part, as the eukaryotic replicative DNA helicase (3,4). Phosphorylation and ubiquitination of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis (5-7). MCM proteins are removed during DNA replication, causing chromatin to become unlicensed, inhibiting Pre-RC reformation. In addition to DNA polymerase, initiation of DNA replication requires a pair of primase subunits. DNA Primase activity catalyzes de novo synthesis of an RNA/DNA primer (initiator DNA) on the leading and lagging strands, while polymerase activity extends the initiator DNA (8). The 48 and 58 kDa primase subunits cooperate in the synthesis of small RNA primers. p48 is the catalytically active subunit (9), while p58 couples p48 to the polymerase to allow the transfer of primers to the active site. The p58 subunit may also play a role in regulation of primer length (10,11). Once replication is initiated, Proliferating Cell Nuclear Antigen (PCNA) serves as an accessory factor for DNA polymerases delta and epsilon, acting to tether these polymerases to template DNA during replication. Interactions of PCNA with DNA polymerases increase the processivity of leading strand synthesis. PCNA, a member of DNA sliding clamp family, is a homotrimeric ring complex that encircles and slides along the DNA double helix as the replication fork progresses (12). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA and regulate DNA synthesis. PCNA is loaded onto the DNA in an ATP-dependent manner by a multiprotein clamp loader, Replication Factor C (RFC) (13). RFC, in turn, associates with DNA via interactions with the single-stranded DNA binding protein complex, Replication Protein A (RPA). The canonical RPA complex is heterotrimeric and composed of RPA1 (RPA70), RPA2 (RPA32), and RPA3 (RPA14) subunits. RPA recognizes and stabilizes single stranded DNA in repair processes and DNA recombination, and plays a role in replication (14-17).

1.  Okuno, Y. et al. (2001) EMBO J 20, 4263-77.

2.  McNairn, A.J. et al. (2005) Exp Cell Res 308, 345-56.

3.  Forsburg, S.L. (2004) Microbiol Mol Biol Rev 68, 109-31.

4.  Johnson, A. and O'Donnell, M. (2005) Annu Rev Biochem 74, 283-315.

5.  Charych, D.H. et al. (2008) J Cell Biochem 104, 1075-86.

6.  Masai, H. et al. (2006) J Biol Chem 281, 39249-61.

7.  Lin, D.I. et al. (2008) Proc Natl Acad Sci USA 105, 8079-84.

8.  Shiratori, A. et al. (1995) Genomics 28, 350-3.

9.  Copeland, W.C. (1997) Protein Expr Purif 9, 1-9.

10.  Copeland, W.C. and Wang, T.S. (1993) J Biol Chem 268, 26179-89.

11.  Arezi, B. and Kuchta, R.D. (2000) Trends Biochem Sci 25, 572-6.

12.  Bowman, G.D. et al. (2004) Nature 429, 724-30.

13.  Zhang, G. et al. (1999) Proc Natl Acad Sci U S A 96, 1869-74.

14.  Sakaguchi, K. et al. (2009) FEBS J 276, 943-63.

15.  Zou, Y. et al. (2006) J Cell Physiol 208, 267-73.

16.  Wold, M.S. (1997) Annu Rev Biochem 66, 61-92.

17.  Binz, S.K. et al. DNA Repair (Amst) 3, 1015-24.

Entrez-Gene Id 81620 , 4171 , 4172 , 4176 , 5111 , 5558 , 6117
Swiss-Prot Acc. Q9H211 , P49736 , P25205 , P33993 , P12004 , P49643 , P27694

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.