Upstream / Downstream

Explore pathways related to this product.

Our U.S. Offices Are Closed

Our U.S. offices are closed in observance of Thanksgiving. We will reopen on Monday, December 1st.

Thank you for your patience.

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

We recommend the following alternatives

        H M R Mk
        H M R Mk
REACTIVITY SENSITIVITY MW (kDa) SOURCE
95-140 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Sec31A Antibody recognizes endogenous levels of multiple isoforms of total Sec31A protein, ranging in sizes of 95-140 kDa.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro810 of human Sec31A protein. Antibodies are purified by protein A and peptide affinity chromatography.

The coat protein complex II (COPII) is composed of five cytosolic proteins and includes the Sec23/24 complex, the Sec13/31 complex, and Sar1. The COPII coat is located at the ER/Golgi interface and is involved in transport of newly synthesized proteins from the ER to the Golgi apparatus (1). COPII formation is initiated through the binding of the activated G protein, Sar1, to the Sec23/24 complex to form a pre-budding complex that directly binds target molecules (1-3). This pre-budding complex further recruits Sec13/31 to form mature COPII coat (4,5). The Sec31 subunit of COPII coat interacts with Sec13 at the ER exit and is required for both vesicle formation and ER-Golgi transport. Two isoforms of human Sec31 have been identified, Sec31A and Sec31B, which share a sequence homology of 47.3% (6-8). Sec31A is ubiquitously expressed in tissues and organs, whereas Sec31B is enriched in brain and testis (7,8). In classical Hodgkin lymphoma, a novel fusion of Jak2 with Sec31A renders Jak2 constitutively active and subject to Jak2 inhibitor effects (9).


Multiple isoforms of Sec31A protein have been reported (10).


1.  Aridor, M. et al. (1998) J Cell Biol 141, 61-70.

2.  Miller, E.A. et al. (2003) Cell 114, 497-509.

3.  Mossessova, E. et al. (2003) Cell 114, 483-95.

4.  Barlowe, C. et al. (1994) Cell 77, 895-907.

5.  Bi, X. et al. (2007) Dev Cell 13, 635-45.

6.  Shugrue, C.A. et al. (1999) J Cell Sci 112 ( Pt 24), 4547-56.

7.  Tang, B.L. et al. (2000) J Biol Chem 275, 13597-604.

8.  Stankewich, M.C. et al. (2006) J Cell Sci 119, 958-69.

9.  Van Roosbroeck, K. et al. (2011) Blood 117, 4056-64.

10.  Townley, A.K. et al. (2008) J Cell Sci 121, 3025-34.


Entrez-Gene Id 22872
Swiss-Prot Acc. O94979


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Tween® is a registered trademark of ICI Americas, Inc.