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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-Rac1/cdc42 (Ser71) Antibody 2461 40 µl
Western Blotting
H 28 Rabbit 
Rac1/Cdc42 Antibody 4651 40 µl
Western Blotting
H M R Mk 21 Rabbit 
WAVE-2 (D2C8) XP® Rabbit mAb 3659 40 µl
Western Blotting Immunoprecipitation Immunofluorescence
H M R Mk 80 Rabbit IgG
Profilin-1 (C56B8) Rabbit mAb 3246 40 µl
Western Blotting
H M R Hm Mk Dg 15 Rabbit IgG
ARP2 Antibody 3128 40 µl
Western Blotting
H M R Hm Mk Dm 44 Rabbit 
ARP3 Antibody 4738 40 µl
Western Blotting
H Mk 47 Rabbit 
N-WASP (30D10) Rabbit mAb 4848 40 µl
Western Blotting Immunoprecipitation
H M R Hm Mk B 65 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 

Product Description

The Actin Nucleation and Polymerization Antibody Sampler Kit provides an economical means to evaluate the presence and status of actin nucleation and polymerization. The kit contains enough primary antibody to perform four western blots per primary.


Specificity / Sensitivity

ARP2 Antibody recognizes endogenous levels of total ARP2 protein. ARP3 Antibody recognizes endogenous levels of total ARP3 protein. This antibody does not cross-react with endogenous ARP2. Profilin-1 (C56B8) Rabbit mAb recognizes endogenous levels of total profilin-1 protein. This antibody may cross-react with profilin-2. Phospho-Rac1/cdc42 (Ser71) Antibody recognizes endogenous levels of Rac1/cdc42 only when phosphorylated at serine 71. This antibody may also detect phospho-RhoA (Ser73). Rac1/Cdc42 Antibody recognizes endogenous levels of total rac1 and cdc42 proteins. Based on sequence similarities, this antibody may also detect rac2 and rac3; this antibody does not cross-react with RhoA, RhoB, or RhoC. N-WASP (30D10) Rabbit mAb recognizes endogenous levels of total N-WASP protein. This antibody does not cross-react wth the hematopietic protein, WASP. WAVE-2 (D2C8) XP® Rabbit mAb recognizes endogenous levels of total WAVE-2 protein.


Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human profilin-1 protein, the sequence of human N-WASP protein, or central residues of human WAVE-2 protein.

Activation state specific polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser71 of human Rac1/cdc42 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino termini of human rac1 and cdc42 proteins, the sequence of human ARP2 protein, or the amino terminus of human ARP3 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Actin nucleation is the process of forming new actin filaments and is necessary to stimulate actin polymerization. Actin polymerization is vital for cell motility, cell division, and cell adhesion. Rac and Cdc42, members of the Rho-GTPase family, play key roles in actin dynamics, membrane trafficking, transcriptional regulation, cell growth, and development (1). GTP binding stimulates the activity of Rac/Cdc42, and the hydrolysis of GTP to GDP through the intrinsic GTPase activity or Rac/Cdc42, rendering it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). Another level of regulation is achieved through binding of RhoGDI, a guanine dissociation inhibitor, which retains Rho family GTPases, including Rac and Cdc42, in their inactive GDP-bound state (2,3). Hematopoietic WASP and ubiquitously expressed N-WASP are autoinhibited in unstimulated cells. Upon stimulation they are activated by Cdc42, which relieves the autoinhibition in conjunction with phosphatidyl inositol 4,5-bisphosphate (4). Three WAVE (Wasf, SCAR) family proteins are similar in sequence to WASP and N-WASP, but lack the WASP/N-WASP autoinhibition domains and are indirectly activated by Rac (4). WAVE-2 is widely distributed, while WAVE-1 and WAVE-3 are strongly expressed in the brain (5). The highly conserved ARP2/3 complex is an important actin nucleation protein complex consisting of ARP2, ARP3, and ARPC1-5. The ARP2/3 complex promotes branching of existing actin filaments and formation of daughter filaments following activation by nucleation-promoting factors, such as WASP/WAVE or cortactin (6). Profilins are conserved actin binding proteins that affect the rate of actin polymerization by binding actin monomers and promoting exchange of ADP for ATP (reviewed in 7). Profilins bind to proteins involved in the regulation of actin dynamics including paladin (8), dynamin-I (9), VASP (10), and N-WASP (11).


1.  Millard, T.H. et al. (2004) Biochem J 380, 1-17.

2.  Suetsugu, S. et al. (1999) Biochem Biophys Res Commun 260, 296-302.

3.  Wennerberg, K. and Der, C.J. (2004) J. Cell Sci. 117, 1301-1312.

4.  Bernards, A. and Settleman, J. (2004) Trends Cell Biol 14, 377-85.

5.  Goley, E.D. and Welch, M.D. (2006) Nat Rev Mol Cell Biol 7, 713-26.

6.  Rossman, K.L. et al. (2005) Nat Rev Mol Cell Biol 6, 167-80.

7.  Witke, W. (2004) Trends Cell Biol 14, 461-9.

8.  Boukhelifa, M. et al. (2006) FEBS J 273, 26-33.

9.  Gareus, R. et al. (2006) J Biol Chem 281, 2803-11.

10.  Reinhard, M. et al. (1995) EMBO J 14, 1583-9.

11.  Suetsugu, S. et al. (1998) EMBO J 17, 6516-26.


Entrez-Gene Id 10097, 10096, 998, 8976, 5216, 5879, 10163
Swiss-Prot Acc. P61160, P61158, P60953, O00401, P07737, P63000, Q9Y6W5


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.