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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Desmin (D93F5) XP® Rabbit mAb 5332 40 µl
H M R 53 Rabbit IgG
Keratin 17 (D73C7) Rabbit mAb 4543 40 µl
H M R 48 Rabbit IgG
Pan-Keratin (C11) Mouse mAb 4545 40 µl
H R Mk 46-58 Mouse IgG1
β-Tubulin (9F3) Rabbit mAb 2128 40 µl
H M R Mk Z B 55 Rabbit IgG
Vimentin (D21H3) XP® Rabbit mAb 5741 40 µl
H M R Mk 57 Rabbit IgG
β-Actin (D6A8) Rabbit mAb 8457 40 µl
H M R Mk 45 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
All Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
All Horse 

Product Description

The Cytoskeletal Marker Antibody Sampler Kit provides an economical means to evaluate the presence and status of select cytoskeleton associated proteins. The kit provides enough primary antibodies to perform four western blots per primary antibody.


Specificity / Sensitivity

Desmin (D93F5) XP® Rabbit mAb, Keratin 17 (D73C7) Rabbit mAb, β-Tubulin (9F3) Rabbit mAb, and Vimentin (D21H3) XP® Rabbit mAb, and B-Actin (D68) Rabbit mAb recognize endogenous levels of total respective target proteins. Pan-Keratin (C11) Mouse mAb detects endogenous levels of total keratins 4, 5, 6, 8, 10, 13, and 18. This antibody does not cross-react with other keratins.


Source / Purification

Rabbit monoclonal antibodies are produced by immunizing animals with a synthetic peptide cooresponding to residues near the amino terminus of human β-actin protein, carboxy terminal residues of human desmin protein, residues near the carboxy terminus of human keratin 17 protein, the amino terminus of human β-tubulin protein, or residues surrounding Arg45 of human vimentin protein. Mouse monoclonal antibody is produced by immunizing animals with a cytoskeleton preparation from A-431 cells.

The cytoskeleton consists of three different types of cystosolic fibers: microtubules, microfilaments (actin) and intermediate filaments. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). Major types of intermediate filaments are distinguished in part by the tissue in which they are expressed, for example; cytokeratins (epithelial cells), vimentin (mesenchyme origin), and desmin (skeletal, visceral and certain vascular smooth muscle cells) (2). Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form intermediate filaments (3). Research studies have demonstrated that vimentin is present in sarcomas, but not carcinomas, and its expression is examined relative to other markers in order to distinguish between the two forms of neoplasm (4). Desmin is a myogenic marker expressed in early development that forms a network of filaments that extends across the myofibril and surrounds Z discs (5). α/β-tubulin heterodimers form the tubulin subunit that comprises the microtubule building block (6).

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1.  Herman, I.M. (1993) Curr Opin Cell Biol 5, 48-55.

2.  Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51.

3.  Moll, R. et al. (1982) Cell 31, 11-24.

4.  Westermann, S. and Weber, K. (2003) Nat. Rev. Mol. Cell Biol. 4, 938 -947.

5.  Leader, M. et al. (1987) Histopathology 11, 63-72.

6.  Li, Z. et al. (1996) Dev Biol 175, 362-6.


Entrez-Gene Id 60, 1674, 3872, 3875, 3853, 3854, 3858, 3860, 3851, 3852, 286887, 3856, 203068, 7431
Swiss-Prot Acc. P60709, P17661, Q04695, P05783, P02538, P04259, P13645, P13646, P19013, P13647, P48668, P05787, P07437, P08670


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.