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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Munc18-1 (D4O6V) Rabbit mAb 13414 40 µl
Western Blotting
H M R 70 Rabbit IgG
Syntaxin 6 (C34B2) Rabbit mAb 2869 40 µl
Western Blotting Immunoprecipitation Immunofluorescence
H M R 32 Rabbit IgG
SNAP25 (D9A12) Rabbit mAb 5309 40 µl
Western Blotting Immunoprecipitation
H M R 25 Rabbit IgG
NSF (D31C7) XP® Rabbit mAb 3924 40 µl
Western Blotting Immunoprecipitation Immunofluorescence
H M R Mk 78 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 

Product Description

The Synaptic Vesicle Antibody Sampler Kit provides an economical means of evaluating proteins involved in synaptic vesicle fusion and membrane trafficking. The kit contains enough primary and secondary antibodies to perform four western miniblot experiments with each antibody.


Specificity / Sensitivity

Munc18-1 (D4O6V) Rabbit mAb recognizes endogenous levels of total Munc18-1 protein. NSF (D31C7) XP® Rabbit mAb detects endogenous levels of total NSF protein. SNAP25 (D9A12) Rabbit mAb detects endogenous levels of total SNAP25 protein. Syntaxin 6 (C34B2) Rabbit mAb detects endogenous levels of total syntaxin 6 protein.


Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr157 of human Munc18-1 protein, residues surrounding Tyr140 of mouse syntaxin 6 protein, residues surrounding Gln116 of human SNAP25 protein, or residues surrounding Leu524 of human NSF protein.

Fusion of a vesicle to its target membrane is a universal process in eukaryotic cells for proper cellular organization and function. Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1,2). SNAP25 forms a core complex with the SNARE proteins syntaxin and synaptobrevin to mediate synaptic vesicle fusion with the plasma membrane during Ca2+-dependent exocytosis (3). Syntaxin 6 is a ubiquitously expressed S25C family member of the SNARE proteins (4,5). Munc18-1 acts as a molecular chaperone for syntaxin-1, allowing for formation of the SNARE complex at the plasma membrane (6). Following membrane fusion, the α-SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the α-SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (7,8). The ATPase activity of NSF induces a conformational change in the α-SNAP/SNARE complex that leads to its dissociation from the membrane, membrane fusion, and eventual recycling of the SNARE complex for subsequent membrane fusion (7,8).


1.  Ungermann, C. and Langosch, D. (2005) J Cell Sci 118, 3819-28.

2.  Bock, J.B. et al. (2001) Nature 409, 839-41.

3.  Leabu, M. J Cell Mol Med 10, 423-7.

4.  Salaün, C. et al. (2004) Biochim Biophys Acta 1693, 81-9.

5.  Medine, C.N. et al. (2007) J Cell Sci 120, 4407-15.

6.  May, A.P. et al. (2001) J Biol Chem 276, 21991-4.

7.  Dalal, S. et al. (2004) Mol Biol Cell 15, 637-48.

8.  Bock, J.B. et al. (1996) J Biol Chem 271, 17961-5.


Entrez-Gene Id 6812, 4905, 6616, 10228
Swiss-Prot Acc. P61764, P46459, P60880, O43752


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.