Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

To get local purchase information on this product, click here

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
AIF (D39D2) XP® Rabbit mAb 5318 40 µl
Western Blotting Immunoprecipitation Immunofluorescence
H M R Mk 67 Rabbit IgG
EEA1 (C45B10) Rabbit mAb 3288 40 µl
Western Blotting Immunoprecipitation Immunofluorescence
H M R 170 Rabbit IgG
LAMP1 (D2D11) XP® Rabbit mAb 9091 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence
H Mk 42 (non-glycosylated), 90-120 (glycosylated) Rabbit IgG
PDI (C81H6) Rabbit mAb 3501 40 µl
Western Blotting Immunohistochemistry Immunofluorescence
H M R Mk 57 Rabbit 
RCAS1 (D2B6N) XP® Rabbit mAb 12290 40 µl
Western Blotting Immunoprecipitation Immunofluorescence Flow Cytometry
H M R 32 Rabbit IgG

Product Description

The Organelle Localization IF Antibody Sampler Kit provides an economical means for identification of cellular organelles by fluorescence immunocytochemistry (IF-IC). This kit includes enough primary antibody to perform at least twenty IF-IC tests or four western blots with each antibody.


Specificity / Sensitivity

Each antibody in the Organelle Localization IF Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Each antibody has been validated for IF-IC and stains the organelles indicated above. Expression of these proteins may vary in different cells and tissues. Please see www.cellsignal.com for additional specificity/sensitivity information for individual kit components.


Source / Purification

Rabbit monoclonal antibodies are produced by immunizing animals with a recombinant protein fragment of human LAMP1 protein, or with a synthetic peptide corresponding to residues surrounding Ala520 of human AIF protein, residues surrounding Ser70 of human EEA1 protein, the sequence of human PDI protein, or residues surrounding Gly147 of human RCAS1 protein.

Knowledge of the subcellular location of a protein may reveal its potential role in a variety of cellular processes. One can confirm the subcellular location of a marker that colocalizes with one of the organelle-specific antibodies in this kit. While these antibodies serve as powerful tools for immunofluorescence, they may also be used as western blot controls for fractionated cell lysates.


Entrez-Gene Id 8411, 3916, 9131, 5034, 9166
Swiss-Prot Acc. Q15075, P11279, O95831, P07237, O00559


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
DRAQ5® is a registered trademark of Biostatus Limited.
MitoTracker® is a registered trademark of Molecular Probes, Inc.
U.S. Patent No. 5,675,063.