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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
CD44 (156-3C11) Mouse mAb 3570 40 µl
H 80 Mouse IgG2a
Cyclin D1 (92G2) Rabbit mAb 2978 40 µl
H M R 36 Rabbit IgG
c-Jun (60A8) Rabbit mAb 9165 40 µl
H M R Mk 43, 48 Rabbit IgG
LEF1 (C12A5) Rabbit mAb 2230 40 µl
H M R 25-58 Rabbit IgG
Met (D1C2) XP® Rabbit mAb 8198 40 µl
H 140, 170 Rabbit IgG
MMP-7 (D4H5) XP® Rabbit mAb 3801 40 µl
M R 28, 20-22 Rabbit 
c-Myc (D84C12) XP® Rabbit mAb 5605 40 µl
H M R 57-65 Rabbit IgG
TCF1 (C63D9) Rabbit mAb 2203 40 µl
H M 48, 50 Rabbit IgG
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
All Horse 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
All Goat 

Product Description

The Wnt/β-Catenin Activated Targets Antibody Sampler Kit provides an economical means to investigate target proteins of the Wnt/β-Catenin signaling pathway. This kit contains enough primary antibody to perform four western blots per primary.

Specificity / Sensitivity

Each antibody in the Wnt/β-Catenin Activated Targets Antibody Sampler Kit detects its respective target at endogenous levels. LEF1 (C12A5) Rabbit mAb does not recognize the dominant negative forms of LEF1 generated by an alternative promoter. c-Myc (D84C12) XP® Rabbit mAb is not recommended for detection of Myc-tagged fusion proteins. TCF1 (C63D9) Rabbit mAb does not recognize the dominant negative isoforms of TCF1 lacking the amino-terminal β-catenin binding domain and does not cross-react with LEF1.

Source / Purification

Rabbit monoclonal antibodies are produced by immunizing animals with a GST-c-Jun protein corresponding to the amino-terminal sequence of human c-Jun protein or synthetic peptides corresponding to residues near the amino terminus of c-Myc protein, residues surrounding Pro82 of human LEF1 protein, residues surrounding Ile264 of mouse MMP-7 protein, residues surrounding Pro95 of human TCF1 protein, residues near the carboxy terminus of human Met protein, or residues near the carboxy-terminus of human cyclin D1 protein.

The mouse monoclonal antibody is produced by immunizing BALB/c mice with stimulated human leukocytes for CD44.

The Wnt family includes several secreted glycoproteins that play important roles in animal development. β-catenin is a key downstream effector of the Wnt signaling pathway that research studies have shown is implicated in early embryonic development and tumorigenesis in vertebrates (1-3). Following binding of Wnt family proteins to the Frizzled receptor, β-catenin translocates to the nucleus where it interacts with LEF1 and TCF1 to activate canonical targets (4). Accepted canonical targets include CD44, cyclin D1, c-Jun, c-Myc, Met, and MMP-7 (5-11).

1.  Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.

2.  Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.

3.  Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.

4.  Brantjes, H. et al. (2002) Biol Chem 383, 255-61.

5.  Zeilstra, J. et al. (2008) Cancer Res 68, 3655-61.

6.  Shtutman, M. et al. (1999) Proc Natl Acad Sci U S A 96, 5522-7.

7.  Mann, B. et al. (1999) Proc Natl Acad Sci U S A 96, 1603-8.

8.  Wilkins, J.A. and Sansom, O.J. (2008) Cancer Res 68, 4963-6.

9.  Boon, E.M. et al. (2002) Cancer Res 62, 5126-8.

10.  Brabletz, T. et al. (1999) Am J Pathol 155, 1033-8.

11.  Clevers, H. and Nusse, R. (2012) Cell 149, 1192-205.

Entrez-Gene Id 960, 595, 3725, 51176, 4233, 4316, 4609, 6932
Swiss-Prot Acc. P16070, P24385, P05412, Q9UJU2, P08581, P09237, P01106, P36402

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.