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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
COX IV (3E11) Rabbit mAb 4850 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H R Mk Z B Pg 17 Rabbit IgG
Cytochrome c (136F3) Rabbit mAb 4280 40 µl
Western Blotting Immunohistochemistry
H M R Mk 14 Rabbit IgG
HSP60 (D6F1) XP® Rabbit mAb 12165 40 µl
Western Blotting Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Hm Mk X Z B Pg 60 Rabbit IgG
PHB1 Antibody 2426 40 µl
Western Blotting Immunoprecipitation
H M R Mk 32 Rabbit 
Pyruvate Dehydrogenase (C54G1) Rabbit mAb 3205 40 µl
Western Blotting Immunohistochemistry
H M R Mk 43 Rabbit IgG
SDHA (D6J9M) XP® Rabbit mAb  11998 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence
H M R Hm Mk 70 Rabbit IgG
SOD1 (71G8) Mouse mAb 4266 40 µl
Western Blotting Immunoprecipitation
H 18 Mouse IgG1
VDAC (D73D12) Rabbit mAb 4661 40 µl
Western Blotting
H M R Mk 32 Rabbit IgG
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
Western Blotting
All Horse 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 

Product Description

The Mitochondrial Marker Antibody Sampler Kit provides an economical means to evaluate relevant mitochondial proteins. This kit contains enough primary antibody to perform four western blots per primary.


Specificity / Sensitivity

Each antibody in the Mitochondrial Marker Antibody Sampler Kit recognizes endogenous levels of total respective protein. SOD1 (71G8) Mouse mAb does not cross-react with other related proteins.


Source / Purification

Rabbit monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human COX IV protein, residues 67-78 of human cytochrome c protein, Trp68 of human HSP60 protein, the sequence of human pyruvate dehydrogenase protein, Gly166 or human SDHA protein, or residues near the amino terminus of human VDAC-1 protein. Mouse monoclonal antibody is produced by immunizing animals with full-length recombinant human SOD1 protein.

Polyclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser252 of human PHB1 protein. Polyclonal antibody is purified by protein A and peptide affinity chromatography.

The Mitochondrial Marker Antibody Sampler Kit contains a variety of antibodies directed against established mitochondrial proteins. Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1). Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to the mitochondrial intermembrane space (2). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (3). In the mitochondria, prohibitins (PHB1) mainly exist as membrane-bound ring complexes and function as chaperones maintaining mitochondrial protein stability during protein synthesis and transportation (4). In mammalian cells, the pyruvate dehydrogenase complex is located in the mitochondrial matrix (5). Succinate dehydrogenase (SDH), also known as Complex II or succinate quinone oxidoreductase, is a key component of the citric acid cycle and the electron transport chain (6). SOD1 is ubiquitously expressed and is localized in the cytosol, nucleus, and mitochondrial intermembrane space (7). Voltage-dependent anion channel (VDAC), ubiquitously expressed and located in the outer mitochondrial membrane, is generally thought to be the primary means by which metabolites diffuse in and out of the mitochondria (8).


1.  Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466.

2.  Schagger H.H. et al. (2002) Biochem. Biophys. Acta. 1555, 154-159.

3.  Oyedotun, K.S. and Lemire, B.D. (2004) J Biol Chem 279, 9424-31.

4.  Jindal, S. et al. (1989) Mol. Cell Biol. 9, 2279-2283.

5.  Craigen, W.J. and Graham, B.H. (2008) J Bioenerg Biomembr 40, 207-12.

6.  Tatsuta, T. et al. (2005) Mol Biol Cell 16, 248-59.

7.  Strumiło, S. (2005) Acta Biochim Pol 52, 759-64.

8.  Sherman, L. et al. (1983) Proc Natl Acad Sci U S A 80, 5465-9.


Entrez-Gene Id 1327, 54205, 3329, 5160, 5245, 6389, 6647, 7416
Swiss-Prot Acc. P13073, P99999, P10809, P08559, P35232, P31040, P00441, P21796


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.