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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
α-Amylase (D55H10) XP® Rabbit mAb 3796 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry
H R 58 Rabbit IgG
CD31 (PECAM-1) (89C2) Mouse mAb 3528 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H 130 Mouse IgG2b
Keratin 17/19 (D32D9) XP® Rabbit mAb 3984 40 µl
Western Blotting Immunohistochemistry
H M R Mk 48/41 Rabbit IgG
Pdx1 (D59H3) XP® Rabbit mAb 5679 40 µl
Western Blotting Immunoprecipitation Immunofluorescence
H R 42 Rabbit IgG
PLA2G1B Antibody 8962 40 µl
Western Blotting
H 16 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
Western Blotting
All Horse 

Product Description

The Pancreatic Marker Antibody Sampler Kit provides an economical means to evaluate relevant pancreatic proteins. This kit contains enough primary antibody to perform four western blots per primary.


Specificity / Sensitivity

Each antibody in the Pancreatic Marker Antibody Sampler Kit recognizes endogenous levels of total respective protein. CD31 (PECAM-1) (89C2) Mouse mAb does not cross-react with other related proteins.


Source / Purification

Rabbit monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human α-amylase, residues near the amino terminus of human keratin 17 and human keratin 19, or a synthetic peptide corresponding to residues surrounding Val274 of human Pdx1 protein. Mouse monoclonal antibody is produced by immunizing animals with recombinant human CD31 protein.

Polyclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala131 of human PLA2G1B protein.

The Pancreatic Marker Antibody Sampler Kit contains a variety of antibodies directed against established pancreatic proteins. α-Amylase catalyzes the cleavage of 1, 4-α-D-glucosidic bonds in oligosaccharides and polysaccharides (1). The enzyme is normally produced and secreted in salivary glands (salivary α-amylase or AMY1) and pancreas (pancreatic α-amylase or AMY2A) (1). CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells, and modulates cell adhesion, endothelial cell migration, and angiogenesis (2). Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (3,4). The pancreatic duodenal homeobox gene-1 (Pdx1) is a transcription factor that contributes to pancreas development, pancreatic β-cell differentiation, and mature β-cell function (5,6). It plays an essential role in the commitment of endoderm to a pancreatic and later β-cell phenotype (6,7). In the mature pancreas, Pdx1 expression is more restricted to the pancreatic β-cells (7), where it promotes the expression of genes important for β-cell functions, such as insulin, glucokinase, and Glut2 (8-10). Phospholipase A2 (PLA2) is a superfamily of enzymes that hydrolyze glycero-3-phosphocholines and release fatty acids and lysophospholipids (11). PLA2G1B is a member of this superfamily in the 1B group that is expressed most highly in the pancreatic acinar cells (12). Evidence suggests that PLA2G1B plays a role in the absorption and storage of extra energy as fats are metabolized (11,12).


1.  Tomita, N. et al. (1988) Cancer Res 48, 3292-6.

2.  Moll, R. et al. (1982) Cell 31, 11-24.

3.  Labonté, E.D. et al. (2010) FASEB J 24, 2516-24.

4.  Newman, P.J. (1997) J Clin Invest 99, 3-8.

5.  Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.

6.  Cash, J.G. et al. (2011) Int J Obes (Lond) 35, 877-81.

7.  Kaneto, H. et al. (2008) Endocr J 55, 235-52.

8.  Habener, J.F. et al. (2005) Endocrinology 146, 1025-34.

9.  Guz, Y. et al. (1995) Development 121, 11-8.

10.  Ohlsson, H. et al. (1993) EMBO J 12, 4251-9.

11.  Watada, H. et al. (1996) Diabetes 45, 1826-31.

12.  Waeber, G. et al. (1996) Mol Endocrinol 10, 1327-34.


Entrez-Gene Id 280, 3872, 3880, 3651, 5175, 5319
Swiss-Prot Acc. P19961, Q04695, P08727, P52945, P16284, P04054


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.