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To Purchase # 8686S

8686S 1 Kit (4 x 40 µl) $329.00
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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
CBP (D6C5) Rabbit mAb 7389 40 µl
H M R Mk 300 Rabbit IgG
Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody 4771 40 µl
H M Mk 300 Rabbit 
PCAF (C14G9) Rabbit mAb 3378 40 µl
H M R Mk 93 Rabbit IgG
GCN5L2 (C26A10) Rabbit mAb 3305 40 µl
H M R Mk 94 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
All Goat 

Product Description

The Lysine Acetyltransferase Antibody Sampler Kit provides an economical means to examine several lysine acetyltrasferases, including: Acetyl-CBP, CBP, GCN5L2, and PCAF. The kit contains enough primary antibody to perform four western blots per primary.

Specificity / Sensitivity

Each antibody in the Lysine Acetyltransferase Antibody Sampler Kit recognizes endogenous levels of respective target protein. The antibodies do not cross-react with other family members.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human CBP protein, human GCN5L2 protein, or human PCAF protein. Polyclonal antibodies are produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding Lys1535 of human CBP. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

CREB-binding protein (CBP) and p300 are highly conserved and functionally related transcriptional co-activators that associate with transcriptional regulators and signaling molecules, integrating multiple signal transduction pathways with the transcriptional machinery (1,2). CBP/p300 also contain histone acetyltransferase (HAT) activity, allowing them to acetylate histones and other proteins (2). The role of acetylation of CBP/p300 is of particular interest (2,3). Acetylation of p300 at Lys1499 has been demonstrated to enhance its HAT activity and affect a wide variety of signaling events (4). p300/CBP-associated factor (PCAF), also known as lysine acetyl-transferase 2B (KAT2B) (5), and General Control of Amino Acid Synthesis Yeast Homolog Like 2 (GCN5L2) (6) are transcriptional adaptor proteins in addition to HATs. PCAF functions as the catalytic subunit of the PCAF transcriptional co-activator complex (5). GCN5L2 functions as the catalytic subunit of the STAGA and TFTC transcription coactivator complexes (6). PCAF and GCN5L2 acetylate histone H3 at Lys14 and histone H4 at Lys8, both of which contribute to gene activation by modulating chromatin structure and recruiting additional co-activator proteins that contain acetyl-lysine binding bromo-domains (7,8). PCAF also acetylates non-histone proteins including transcriptional activators (p53, E2F1, MyoD) and general transcription factors (TFIIEβ and TFIIF) (9-12). GCN5L2 also acetylates non-histone proteins such as transcription activators (TAT, c-Myb) (13,14), transcription co-activators (PGC1-α) (15), and nuclear receptors (Steroidogenic Factor 1) (16). Acetylation of these proteins regulates their nuclear localization, protein stability, DNA binding, and co-activator association (13-16).

1.  Goodman, R.H. and Smolik, S. (2000) Genes Dev 14, 1553-77.

2.  Chan, H.M. and La Thangue, N.B. (2001) J Cell Sci 114, 2363-73.

3.  Yuan, L.W. and Giordano, A. (2002) Oncogene 21, 2253-60.

4.  Liu, L. et al. (1999) Mol Cell Biol 19, 1202-9.

5.  Thompson, P.R. et al. (2004) Nat Struct Mol Biol 11, 308-15.

6.  Nagy, Z. and Tora, L. (2007) Oncogene 26, 5341-57.

7.  Candau, R. et al. (1996) Mol Cell Biol 16, 593-602.

8.  Grant, P.A. et al. (1999) J Biol Chem 274, 5895-900.

9.  Bannister, A.J. and Miska, E.A. (2000) Cell Mol Life Sci 57, 1184-92.

10.  Sartorelli, V. et al. (1999) Mol Cell 4, 725-34.

11.  Imhof, A. et al. (1997) Curr Biol 7, 689-92.

12.  Schiltz, R.L. et al. (1999) J Biol Chem 274, 1189-92.

13.  Kiernan, R.E. et al. (1999) EMBO J 18, 6106-18.

14.  Tomita, A. et al. (2000) Oncogene 19, 444-51.

15.  Lerin, C. et al. (2006) Cell Metab 3, 429-38.

16.  Jacob, A.L. et al. (2001) J Biol Chem 276, 37659-64.

Entrez-Gene Id 1387, 2648, 8850, 2033
Swiss-Prot Acc. Q92793, Q92830, Q92831, Q09472

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.