Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Our U.S. Offices Are Closed

Our U.S. offices are closed in observance of Memorial Day. We will reopen on Tuesday, May 31st.

Thank you for your patience.

Questions?

Find answers on our FAQs page.

ANSWERS  

PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

LEARN MORE

We recommend the following alternatives

W IP         M R
    IHC IF     H M R
REACTIVITY SENSITIVITY MW (kDa) SOURCE
37, 75 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Rhodopsin Antibody recognizes endogenous levels of total rhodopsin protein. This antibody may also detect rhodopsin protein at 55 kDa.


Species predicted to react based on 100% sequence homology: Hamster, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro7 of human rhodopsin protein. Antibodies are purified by protein A and peptide affinity chromatography.

Rhodopsin is the photoreceptor in the retinal rods. It is activated by photons, transduces visual information through its cognate G protein, transducin, and is inactivated by arrestin binding (1). Using atomic-force microscopy, rhodopsin was found to be arranged into paracrystalline arrays of dimers in mouse disc membranes (2). Rhodopsin is considered to be the prototype of G protein-coupled receptors (GPCRs), and is the first GPCR for which a crystal structure was solved (3). Research studies have linked mutations in the gene encoding rhodopsin to retinitis pigmentosa (4,5), a disease characterized by retinal degeneration resulting in reduced peripheral vision and night blindness (6).


1.  Arshavsky, V.Y. and Burns, M.E. (2012) J Biol Chem 287, 1620-6.

2.  Fotiadis, D. et al. (2003) Nature 421, 127-8.

3.  Palczewski, K. et al. (2000) Science 289, 739-45.

4.  Rivolta, C. et al. (2002) Hum Mol Genet 11, 1219-27.

5.  Wilson, J.H. and Wensel, T.G. (2003) Mol Neurobiol 28, 149-58.

6.  Hartong, D.T. et al. (2006) Lancet 368, 1795-809.


Entrez-Gene Id 6010
Swiss-Prot Acc. P08100


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

8710
Rhodopsin Antibody