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130 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Spinophilin Antibody recognizes endogenous levels of total spinophilin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala369 of human spinophilin protein. Antibodies are purified by protein A and peptide affinity chromatography.

Spinophilin is an 815 amino acid protein composed of a PDZ domain, 2 actin-binding domains, a receptor- and PP1-binding domain, three coiled-coiled domains, a potential leucine/isoleucine zipper motif, and three potential SH3 domains (1). Spinophilin interacts with a large number of proteins including ion channel components and G protein-coupled receptors (GPCRs). Spinophilin also interacts with actin filaments; phosphorylation of spinophilin at Ser94 and Ser177 disrupts this interaction (2). Spinophilin has been shown to affect GPCR function through two different mechanisms: spinophilin acts as a functional inhibitor of α-2 adrenergic receptor-mediated arrestin signaling by competing with GRK2 binding to the adrenergic receptor (3) and spinophilin facilitates μ-opioid receptor desensitization by promoting receptor endocytosis (4).

1.  Sarrouilhe, D. et al. (2006) Biochimie 88, 1099-113.

2.  Hsieh-Wilson, L.C. et al. (2003) J Biol Chem 278, 1186-94.

3.  Wang, Q. et al. (2004) Science 304, 1940-4.

4.  Charlton, J.J. et al. (2008) Neuron 58, 238-47.

Entrez-Gene Id 84687
Swiss-Prot Acc. Q96SB3

For Research Use Only. Not For Use In Diagnostic Procedures.
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