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Western blot analysis of Elk-1 fusion protein expressed from E. coli with or without phosphorylation by purified Erk2 enzyme, using Phospho-Elk-1 (Ser383) Antibody (upper) or control Elk-1 Antibody #9182 (lower).

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Western blot analysis of Elk-1 fusion protein phosphorylated in vitro with purified Erk2 enzyme, using control Elk-1 Antibody.

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After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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Image
Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-Elk-1 (Ser383) Antibody 9181 100 µl
H 47 Rabbit 
Elk-1 Antibody 9182 100 µl
H M R Z 47 Rabbit 
Elk-1 Control Proteins 9183 150 µl
40 kDa nonphosphorylated, 50 kDa phosphorylated E. Coli 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
All Goat 
Anti-biotin, HRP-linked Antibody 7075 100 µl
Goat 
Biotinylated Protein Ladder Detection Pack 7727 100 µl
 
20X LumiGLO® Reagent and 20X Peroxide 7003 5 ml each
 

Product Usage Information

Protocols

Product Description

The PhosphoPlus® Elk-1 (Ser383) Antibody Kit provides reagents and protocols for the rapid analysis of the phosphorylation status of Elk-1. Bacterially expressed Elk-1 serves as a negative control. The same protein phosphorylated by p42 MAP Kinase (Erk2) serves as a positive control.


Specificity / Sensitivity

Elk-1 Antibody detects endogenous levels of total Elk-1 protein. Phospho-Elk-1 (Ser383) Antibody detects EIk-1 protein only when activated by phosphorylation at serine 383.


Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phosphopeptide corresponding to residues surrounding Ser383 of human Elk-1, or with a synthetic peptide derived from the sequence of human Elk-1. Antibodies are purified by protein A and peptide affinity chromatography.

The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5).


1.  Hill, C.S. and Treisman, R. (1995) Cell 80, 199-211.

2.  Cavigelli, M. et al. (1995) EMBO J. 14, 5957-5964.

3.  Whitmarsh, A.J. et al. (1995) Science 269, 403-407.

4.  Marais, R. et al. (1993) Cell 73, 381-93.

5.  Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24.


Entrez-Gene Id 2002
Swiss-Prot Acc. P19419


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus® is a trademark of Cell Signaling Technology, Inc.
LumiGLO® is a registered trademark of Kirkegaard & Perry Laboratories.