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Western blot analysis of extracts from HeLa, C6 and NIH/3T3 cells, untreated or anisomycin-treated, using Phospho-ATF-2 (Thr71) Antibody.

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Western blot analysis of extracts from 293 and NIH/3T3 cells, untreated or UV-treated, using ATF-2 (20F1) Rabbit mAb.

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After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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Immunohistochemical analysis of paraffin-embedded human breast carcinoma showing nuclear localization, using Phospho-ATF-2 (Thr71) Antibody .

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Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma using ATF-2 (20F1) Rabbit mAb.

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Immunohistchemical analysis of paraffin-embedded human colon carcinoma using Phospho-ATF-2 (Thr71) Antibody.

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Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-ATF-2 (Thr71) Antibody.

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Immunhistochemical analysis of frozen H1650 xenograft using Phospho-ATF-2 (Thr71) Antibody.

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Flow cytometric analysis of Jurkat cells, untreated (blue) or Anisomycin-treated (green), using Phospho-ATF-2 (Thr71) Antibody compared to a nonspecific negative control antibody (red).

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Confocal immunofluorescent analysis of HeLa cells, either untreated (left) or anisomycin-treated (right), using Phospho-ATF-2 (Thr71) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

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Image
Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-ATF-2 (Thr71) Antibody 9221 100 µl
H M R Mk 70 Rabbit 
ATF-2 (20F1) Rabbit mAb 9226 100 µl
H M R Mk 65 to 75 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 40 µl
All Goat 
Anti-biotin, HRP-linked Antibody 7075 1 ml
Goat 
Biotinylated Protein Ladder Detection Pack 7727 1 µl
 
20X LumiGLO® Reagent and 20X Peroxide 7003 5 ml each
 
ATF-2 Control Cell Extracts 9223 50 µl
 

Specificity / Sensitivity

Phospho-ATF-2 (Thr71) Antibody detects endogenous levels of ATF-2 only when phosphorylated at Thr71. It recognizes this site regardless of the phosphorylation state of Thr69. ATF-2 (20F1) Rabbit mAb detects endogenous levels of total ATF-2 protein. Neither antibody cross-reacts with c-Jun, CREB or other transcription factors.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr71 of human ATF-2 (Phospho-ATF-2 (Thr71) Antibody), or with a synthetic peptide corresponding to the amino terminal sequence of human ATF-2 (ATF-2 (20F1) Rabbit mAb). Antibodies are purified by protein A and peptide affinity chromatography.

The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).


1.  Abdel-Hafiz, H.A. et al. (1992) Mol Endocrinol 6, 2079-89.

2.  Gupta, S. et al. (1995) Science 267, 389-93.

3.  van Dam, H. et al. (1995) EMBO J 14, 1798-811.

4.  Livingstone, C. et al. (1995) EMBO J 14, 1785-97.


Entrez-Gene Id 1386
Swiss-Prot Acc. P15336


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus® is a trademark of Cell Signaling Technology, Inc.
LumiGLO® is a registered trademark of Kirkegaard & Perry Laboratories.
U.S. Patent No. 5,675,063.