Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

To get local purchase information on this product, click here

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Western blot analysis of extracts from HeLa, C6 and NIH/3T3 cells, untreated or anisomycin-treated, using Phospho-ATF-2 (Thr71) Antibody.

Learn more about how we get our images

Western blot analysis of extracts from 293 and NIH/3T3 cells, untreated or UV-treated, using ATF-2 (20F1) Rabbit mAb.

Learn more about how we get our images

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Learn more about how we get our images

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Learn more about how we get our images

Immunohistochemical analysis of paraffin-embedded human breast carcinoma showing nuclear localization, using Phospho-ATF-2 (Thr71) Antibody .

Learn more about how we get our images

Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma using ATF-2 (20F1) Rabbit mAb.

Learn more about how we get our images

Immunohistchemical analysis of paraffin-embedded human colon carcinoma using Phospho-ATF-2 (Thr71) Antibody.

Learn more about how we get our images

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-ATF-2 (Thr71) Antibody.

Learn more about how we get our images

Immunhistochemical analysis of frozen H1650 xenograft using Phospho-ATF-2 (Thr71) Antibody.

Learn more about how we get our images

Flow cytometric analysis of Jurkat cells, untreated (blue) or Anisomycin-treated (green), using Phospho-ATF-2 (Thr71) Antibody compared to a nonspecific negative control antibody (red).

Learn more about how we get our images

Confocal immunofluorescent analysis of HeLa cells, either untreated (left) or anisomycin-treated (right), using Phospho-ATF-2 (Thr71) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

Learn more about how we get our images
Image
Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-ATF-2 (Thr71) Antibody 9221 100 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Mk 70 Rabbit 
ATF-2 (20F1) Rabbit mAb 9226 100 µl
Western Blotting Immunoprecipitation Immunohistochemistry
H M R Mk 65 to 75 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 40 µl
Western Blotting
All Goat 
Anti-biotin, HRP-linked Antibody 7075 1 ml
Goat 
Biotinylated Protein Ladder Detection Pack 7727 1 µl
 
20X LumiGLO® Reagent and 20X Peroxide 7003 5 ml each
Western Blotting
 
ATF-2 Control Cell Extracts 9223 50 µl
 

Specificity / Sensitivity

Phospho-ATF-2 (Thr71) Antibody detects endogenous levels of ATF-2 only when phosphorylated at Thr71. It recognizes this site regardless of the phosphorylation state of Thr69. ATF-2 (20F1) Rabbit mAb detects endogenous levels of total ATF-2 protein. Neither antibody cross-reacts with c-Jun, CREB or other transcription factors.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr71 of human ATF-2 (Phospho-ATF-2 (Thr71) Antibody), or with a synthetic peptide corresponding to the amino terminal sequence of human ATF-2 (ATF-2 (20F1) Rabbit mAb). Antibodies are purified by protein A and peptide affinity chromatography.

The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).


1.  Abdel-Hafiz, H.A. et al. (1992) Mol Endocrinol 6, 2079-89.

2.  Gupta, S. et al. (1995) Science 267, 389-93.

3.  van Dam, H. et al. (1995) EMBO J 14, 1798-811.

4.  Livingstone, C. et al. (1995) EMBO J 14, 1785-97.


Entrez-Gene Id 1386
Swiss-Prot Acc. P15336


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus® is a trademark of Cell Signaling Technology, Inc.
LumiGLO® is a registered trademark of Kirkegaard & Perry Laboratories.
U.S. Patent No. 5,675,063.