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Western blot analysis of extracts from 293 or SK-N-MC cells treated with UV (40 mJ/cm2), using Phospho-SAPK/JNK (Thr183/Tyr185) Antibody (upper) or SAPK/JNK Antibody (lower).

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After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-SAPK/JNK (Thr183/Tyr185) Antibody 9251 200 µl
Western Blotting Immunoprecipitation
H M R Hm Mk Dm B Sc 46, 54 Rabbit 
SAPK/JNK Antibody 9252 200 µl
Western Blotting
H M R Hm Mk Z B Sc 46, 54 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 
Anti-biotin, HRP-linked Antibody 7075 250 µl
Western Blotting
All Goat 
20X LumiGLO® Reagent and 20X Peroxide 7003 10 ml
Western Blotting
 
Biotinylated Protein Ladder Detection Pack 7727 250 µl
 
SAPK/JNK Control Cell Extracts 9253 150 µl
 

Product Description

The PhosphoPlus® SAPK/JNK (Thr183/Tyr185) Antibody Kit provides reagents and protocols for the rapid analysis of SAPK/JNK phosphorylation.


Specificity / Sensitivity

Phospho-SAPK/JNK (Thr183/Tyr185) Antibody detects endogenous levels of p46 and p54 SAPK/JNK dually phosphorylated at threonine 183 and tyrosine 185. This antibody does not recognize unphosphorylated SAPK/JNK. This antibody may slightly cross-react with phospho-ERK1/2 or -p38 phosphorylated at homologous residues. SAPK/JNK Antibody detects endogenous levels of total SAPK/JNK protein.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr183/Tyr185 of human SAPK/JNK or with a recombinant human JNK2 fusion protein. Antibodies are purified by protein A and peptide affinity chromatography.

The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5).


1.  Ichijo, H. (1999) Oncogene 18, 6087-93.

2.  Kyriakis, J.M. and Avruch, J. (2001) Physiol Rev 81, 807-69.

3.  Kyriakis, J.M. (1999) J Biol Chem 274, 5259-62.

4.  Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485.

5.  Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.

6.  Davis, R.J. (1999) Biochem Soc Symp 64, 1-12.


Entrez-Gene Id 5599
Swiss-Prot Acc. P45983


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
U.S. Patent No. 5,675,063.