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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
GSK-3α (D80E6) Rabbit mAb 4337 40 µl
Western Blotting Immunoprecipitation
H M R Hm Mk 51 Rabbit IgG
Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb 9316 40 µl
Western Blotting Immunohistochemistry
H M R Mk 51 Rabbit 
Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb 5558 40 µl
Western Blotting Immunoprecipitation Immunofluorescence Flow Cytometry
H M R Hm 46 Rabbit IgG
Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb 8566 40 µl
Western Blotting Immunoprecipitation
H M R Hm Mk 46 GSK-3beta, 51 GSK-3alpha Rabbit IgG
GSK-3β (D5C5Z) XP® Rabbit mAb 12456 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Mk 46 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 

Product Description

The GSK-3 Antibody Sampler Kit contains primary and secondary antibodies to perform four Western blots with each antibody.

Specificity / Sensitivity

Each antibody in the GSK3 Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Phospho-GSK-3ß (Ser9) (D85E12) XP® Rabbit mAb reacts with denatured components of bovine serum, including BSA.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues around Ser9 of human GSK-3ß, residues around Ser21 of human GSK-3α, or to the sequence of human GSK-3α protein or by immunizing animals with recombinant protein specific to the carboxy terminus of human GSK-3ß protein.

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).

1.  Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.

2.  Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.

3.  Cross, D.A. et al. (1995) Nature 378, 785-9.

4.  Nusse, R. (1997) Cell 89, 321-3.

5.  Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.

Entrez-Gene Id 2931, 2932
Swiss-Prot Acc. P49840, P49841

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.