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PhosphoSitePlus® Resource

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REACTIVITY SENSITIVITY MW (kDa) SOURCE
70 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Smad4 Antibody detects endogenous levels of total Smad4 protein.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the residues surrounding Pro278 of human Smad4. Antibodies are purified by protein A and peptide affinity chromatography.

Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the recepter-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Briefly, activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved SSXS motif at the carboxy-terminus of the proteins. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad, Smad4, and together the complex moves to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8).


1.  Heldin, C. H. et al. (1997) Nature 390, 465-71.

2.  Attisano, L. and Wrana, J.L. (1998) Curr. Opin. Cell Biol. 10, 188-94.

3.  Derynck, R. et al. (1998) Cell 95, 737-40.

4.  Massague, J. (1998) Annu. Rev. Biochem. 67, 753-91.

5.  Whitman, . (1998) Genes Dev. 12, 2445-62.

6.  Wrana, J. (2000) Science 23, 1-9.

7.  Attisano, L. and Wrana, J. (2002) Science 296, 1646-7.

8.  Moustakas, A. et al. (2001) J. Cell Sci. 114, 4359-69.


Entrez-Gene Id 4089
Swiss-Prot Acc. Q13485


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

9515
Smad4 Antibody