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PTMScan® Motif Antibody Kits
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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Specificity / Sensitivity

Immobilized Phospho-(Ser/Thr) Akt Substrate Antibody is useful for immunoprecipitation of phosphorylated Akt substrate proteins. This antibody recognizes proteins containing phospho-Ser/Thr preceded by Arg or Lys at positions -5 and -3. It shows some cross-reactivity with phospho-Ser/Thr preceded by Arg or Lys at positions -3 and -2. It does not cross-react with other phospho-Ser/Thr-containing motifs. The antibody is immobilized by conjugation of carbohydrates to cross-linked agarose hydrazide beads. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Species Reactivity: All Species Expected

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a phospho-Akt substrate peptide. Antibodies are purified by protein A and peptide affinity chromatography.

An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (3-5). While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery.

1.  Alessi, D.R. et al. (1996) FEBS Lett 399, 333-8.

2.  Marte, B.M. and Downward, J. (1997) Trends Biochem Sci 22, 355-8.

3.  Jiang, B.H. et al. (2000) Proc Natl Acad Sci USA 97, 1749-53.

4.  Scheid, M.P. and Woodgett, J.R. (2000) Curr Biol 10, R191-4.

5.  Montminy, M. (1997) Annu Rev Biochem 66, 807-22.

6.  Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at For information regarding commercial licensing terms please contact CST Pharma Services Department at

Immobilized Phospho-(Ser/Thr) Akt Substrate Antibody