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PTMScan® Motif Antibody Kits
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Now 10 Assays Per Kit

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REACTIVITY SENSITIVITY MW (kDa) SOURCE
All Endogenous Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Specificity / Sensitivity

Immobilized Phospho-(Ser/Thr) Akt Substrate Antibody is useful for immunoprecipitation of phosphorylated Akt substrate proteins. This antibody recognizes proteins containing phospho-Ser/Thr preceded by Arg or Lys at positions -5 and -3. It shows some cross-reactivity with phospho-Ser/Thr preceded by Arg or Lys at positions -3 and -2. It does not cross-react with other phospho-Ser/Thr-containing motifs. The antibody is immobilized by conjugation of carbohydrates to cross-linked agarose hydrazide beads. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)


Species Reactivity: All Species Expected

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a phospho-Akt substrate peptide. Antibodies are purified by protein A and peptide affinity chromatography.

An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (3-5). While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery.


1.  Montminy, M. (1997) Annu Rev Biochem 66, 807-22.

2.  Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.

3.  Marte, B.M. and Downward, J. (1997) Trends Biochem Sci 22, 355-8.

4.  Jiang, B.H. et al. (2000) Proc Natl Acad Sci USA 97, 1749-53.

5.  Scheid, M.P. and Woodgett, J.R. (2000) Curr Biol 10, R191-4.

6.  Alessi, D.R. et al. (1996) FEBS Lett 399, 333-8.



For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.