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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Raptor (24C12) Rabbit mAb 2280 40 µl
Western Blotting Immunoprecipitation
H M R Mk 150 Rabbit IgG
mTOR (7C10) Rabbit mAb 2983 40 µl
Western Blotting Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Mk 289 Rabbit 
RagB (D18F3) Rabbit mAb 8150 40 µl
Western Blotting Immunoprecipitation
H Mk 40 Rabbit IgG
RagA (D8B5) Rabbit mAb 4357 40 µl
Western Blotting Immunoprecipitation
H M R Mk 30 Rabbit IgG
RagD Antibody 4470 40 µl
Western Blotting
H 50 Rabbit 
RagC (D31G9) XP® Rabbit mAb 5466 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry
H M R Mk 50 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 

Product Description

The Rag Protein Antibody Sampler Kit provides an economical means to evaluate the regulation of mTORC1 signaling by such proteins as Raptor, RagA, RagB, RagC, and RagD. The kit contains enough primary and secondary antibodies to perform four Western blot experiments per primary antibody.


Specificity / Sensitivity

Each antibody in the Rag Protein Antibody Sampler Kit detects endogenous levels of its target protein. RagA (D8B5) Rabbit mAb may also detect endogenous levels of total RagB protein.


Source / Purification

Polyclonal antibody is produced by immunizing animal with synthetic peptide corresponding to residues in the human sequence for RagD proteins. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues of human mTOR, RagA, and Raptor, and corresponding to residues near the amino terminus of human RagB, and RagC protein.

The mTORC1 kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids via signaling through Akt, MAPK, and AMPK pathways (3,4). Recent studies found that the four related GTPases, RagA, RagB, RagC, and RagD, interact with raptor within the mTORC1 complex (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2).


1.  Sancak, Y. et al. (2008) Science 320, 1496-501.

2.  Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.

3.  Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.

4.  Wullschleger, S. et al. (2006) Cell 124, 471-84.


Entrez-Gene Id 2475, 10670, 10325, 64121, 58528, 57521
Swiss-Prot Acc. P42345, Q7L523, Q5VZM2, Q9HB90, Q9NQL2, Q8N122


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.