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9786
Mismatch Repair Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Mismatch Repair Antibody Sampler Kit #9786

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Western blot analysis of extracts of HeLa and NIH/3T3 cells using MSH2 (D24B5) XP® Rabbit mAb.
Western blot analysis of extracts from various cell types using MLH1 (4C9C7) Mouse mAb.
Western blot analysis of extracts from various cell types using MSH6 (L990) Antibody.
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using MSH2 (D24B5) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
MLH1 was immunoprecipitated from HeLa cell lysates using MLH1 (4C9C7) Mouse mAb. Western blot was performed using the same antibody. Lane 1 is 5% input.
Confocal immunofluorescent analysis of HCT-116 cells using MSH6 (L990) Antibody (green). Actin filaments have been labeled using DY-554 phalloidin (red).
Confocal immunofluorescent analysis of HeLa cells using MSH2 (D24B5) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Confocal immunofluorescent analysis of T-47D cells (left, positive) and HCT 116 cells (right, negative) using MLH1 (4C9C7) Mouse mAb (green), DyLight 554 Phalloidin #13054 (red), and DAPI #4083 (blue).

Flow cytometric analysis of HeLa cells using MSH2 (D24B5) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
Flow cytometric analysis of IGROV-1 cells (red) and Jurkat cells (blue) using MLH1 (4C9C7) Mouse mAb.
Inquiry Info.# 9786

Product Description

Mismatch Repair Antibody Sampler Kit provides an economical means to investigate the DNA mismatch repair system within the cell. The kit contains primary and secondary antibodies to perform four western blots with each antibody.

Specificity / Sensitivity

MLH1 (4C9C7) Mouse mAb detects endogenous levels of total MLH1 protein. MSH2 (D24B5) XP® Rabbit mAb detects endogenous levels of total MSH2 protein. MSH6 (L990) Antibody detects endogenous levels of total MSH6 protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with truncated recombinant MBP-MLH1 or by immunizing animals with a synthetic peptide corresponding to residues at the amino terminus of human MSH2. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MLH6 protein. Antibodies are purified using protein A and peptide affinity chromatography.

Background

The DNA mismatch repair system (MMR) repairs post-replication DNA, inhibits recombination between nonidentical DNA sequences, and induces both checkpoint and apoptotic responses following certain types of DNA damage (1). MSH2 (MutS homologue 2) forms the hMutS-α dimer with MSH6 and is an essential component of the mismatch repair process. hMutS-α is part of the BRCA1-associated surveillance complex (BASC), a complex that also contains BRCA1, MLH1, ATM, BLM, PMS2 proteins, and the Rad50-Mre11-NBS1 complex (2). Mutations in MSH6 and other MMR proteins have been found in a large proportion of hereditary nonpolyposis colorectal cancer (Lynch Syndrome), the most common form of inherited colorectal cancer in the Western world (3). Mutations in MSH6 have been shown to occur in glioblastoma in response to temozolomide therapy and to promote temozolomide resistance (4).

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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