Upstream / Downstream

Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
SUZ12 (D39F6) XP® Rabbit mAb 3737 40 µl
H M R Mk 83 Rabbit IgG
Ezh2 (D2C9) XP® Rabbit mAb 5246 40 µl
H M R Mk 98 Rabbit IgG
Ring1A (D2P4D) Rabbit mAb 13069 40 µl
H R 54 Rabbit IgG
RING1B (D22F2) XP® Rabbit mAb 5694 40 µl
H M R Mk 41 Rabbit IgG
Bmi1 (D20B7) XP® Rabbit mAb 6964 40 µl
H Mk 41, 43 Rabbit IgG
Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb 9733 40 µl
H M R Mk 17 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
All Goat 

Product Description

The Polycomb Group Antibody Sampler Kit provides an economical means of evaluating total levels of Polycomb Group Proteins. The kit contains enough primary and secondary antibodies to perform four western mini-blot experiments.


Specificity / Sensitivity

Each antibody in the Polycomb Group Antibody Sampler Kit detects endogenous levels of target proteins.


Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues surrounding Arg354 of human Ezh2, Pro316 of human Ring1A protein, the human SUZ12 protein, the carboxy terminus of human Bmi1 protein, the amino terminus of histone H3 in which Lys27 is tri-methylated, or with recombinant protein specific to the full-length human RING1B protein, respectively.

The polycomb group (PcG) proteins contribute to the maintenance of cell identity, stem cell self-renewal, cell-cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell-cycle arrest (1-4). PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, Eed-Ezh2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. This histone methyltransferase activity requires the Ezh2, Eed, and Suz12 subunits of the complex (5). Methylation of Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinates histone H2A on Lys119 (6). PRC1 is composed of Bmi1 and RING1A (also RING1 or RNF1), both of which act to enhance the E3 ubiquitin ligase activity of an additional catalytic subunit RING1B (also RING2 or RNF2) (7). PcG proteins play an important role in the regulation of cell proliferation and senescence through repression of the p16 INK4A and p19 ARF genes and are required for maintenance of adult hematopoietic and neural stem cells, as well as embryonic stem cells (3,4,8-10).


1.  Boyer, L.A. et al. (2006) Nature 441, 349-53.

2.  Park, I.K. et al. (2003) Nature 423, 302-5.

3.  Molofsky, A.V. et al. (2003) Nature 425, 962-7.

4.  Molofsky, A.V. et al. (2005) Genes Dev 19, 1432-7.

5.  Lee, T.I. et al. (2006) Cell 125, 301-13.

6.  Cao, R. and Zhang, Y. (2004) Mol Cell 15, 57-67.

7.  Wang, H. et al. (2004) Nature 431, 873-8.

8.  Cao, R. et al. (2005) Mol Cell 20, 845-54.

9.  Jacobs, J.J. et al. (1999) Nature 397, 164-8.

10.  Jacobs, J.J. et al. (1999) Genes Dev 13, 2678-90.


Entrez-Gene Id 648, 2146, 8350, 6015, 6045, 23512
Swiss-Prot Acc. P35226, Q15910, P68431, Q06587, Q99496, Q96BD9


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.