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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb 4511 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Mk Mi Pg Sc 43 Rabbit IgG
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb 4370 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Hm Mk Mi Dm Z B Dg Pg Sc 44, 42 Rabbit IgG
Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb 4668 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry
H M R Dm Sc 46, 54 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 
U0126 9903 0.3 mg
 

Product Description

The Phospho-MAPK Family Antibody Sampler Kit provides an economical means of evaluating the phosphorylation state of p38, p44/42, and SAPK/JNK mitogen-activated protein kinases. The kit contains enough primary and secondary antibodies to perform four western blot experiments.


Specificity / Sensitivity

Phospho-p44/42 MAPK, Phospho-SAPK/JNK and Phospho-p38 MAPK Antibodies only recognize the phosphorylated forms of p44/42 MAPK, SAPK/JNK and p38 MAPK, respectively. They do not significantly cross-react with other MAPK family members.


Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides surrounding Thr180/Tyr182 of human p38 MAPK, Thr202/Tyr204 of human p44 MAPK or Thr183/Tyr185 of human SAPK/JNK.

p44/42 MAPK (Erk1/2), SAPK/JNK, and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, differentiation, and control of cellular responses to cytokines and stress. p44/42 MAPK is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine residues (Thr202 and Tyr204 in human Erk1) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). SAPK/JNK and p38 MAPK are activated by inflammatory cytokines and by a wide variety of cellular stresses. Activation of SAPK/JNK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* to activate p38 MAP kinase (1-5).


1.  Lewis, T. S. et al. (1998) Adv. Cancer Res. 74, 49-139.

2.  Garrington, T.P. and Johnson, G.L. (1999) Curr. Opin. Cell. Biol. 11, 211-218.

3.  Schaeffer, H.J. and Weber, M.J. (1999) Mol. Cell. Biol. 19, 2435-2444.

4.  Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485.

5.  Cobb, M.H. (1999) Prog. Biophys. Mol. Biol. 71, 479-500.


Entrez-Gene Id 5595, 5594, 1432, 5600, 5603, 6300, 5599
Swiss-Prot Acc. P27361, P28482, Q16539, Q15759, O15264, P53778, P45983


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.