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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb 4668 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry
H M R Dm Sc 46, 54 Rabbit IgG
Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb 4514 40 µl
Western Blotting Flow Cytometry
H M R Mk 44 Rabbit 
Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb 5112 40 µl
Western Blotting Flow Cytometry
H M R Mk 70 Rabbit IgG
Phospho-c-Jun (Ser63) (54B3) Rabbit mAb 2361 40 µl
Western Blotting Immunohistochemistry
H M R 48 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 

Product Description

The Phospho-SAPK/JNK Pathway Antibody Sampler Kit provides a fast and economical means of evaluating multiple members of the SAPK/JNK pathway as well as their activation state. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.


Specificity / Sensitivity

Each antibody in the Phospho-SAPK/JNK Antibody Sampler Kit recognizes endogenous levels of the phosphorylated form of its specific target.


Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptides corresponding to residues surrounding Ser257 of human SEK1, Thr183/Tyr185 of human SAPK, Thr71 of human ATF-2 or Ser63 of human c-Jun.

The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5).


1.  Ichijo, H. (1999) Oncogene 18, 6087-93.

2.  Kyriakis, J.M. and Avruch, J. (2001) Physiol Rev 81, 807-69.

3.  Kyriakis, J.M. (1999) J Biol Chem 274, 5259-62.

4.  Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485.

5.  Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.

6.  Davis, R.J. (1999) Biochem Soc Symp 64, 1-12.


Entrez-Gene Id 1386, 3725, 6416, 5599
Swiss-Prot Acc. P15336, P05412, P45985, P45983


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.