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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb 4060 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Hm Mk Dm Z B 60 Rabbit IgG
Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb 9234 40 µl
Western Blotting
H M R Mk 70, 85 Rabbit IgG
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb 4858 40 µl
Western Blotting Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Mk Mi Sc 32 Rabbit IgG
Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb 3398 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry
H M R Mk Dm 38 Rabbit IgG
Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb 2855 40 µl
Western Blotting Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Mk Dm 15 to 20 Rabbit IgG
Phospho-eIF4E (Ser209) Antibody 9741 40 µl
Western Blotting
H M R Mk 25 Rabbit 
Rapamycin 9904 2 µg
 
LY294002 9901 0.3 mg
 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 

Product Description

The Translational Control Antibody Sampler Kit provides a fast and economical means of evaluating multiple proteins involved in translational control. The kit contains enough primary and secondary antibody to perform four Western blot experiments, as well as specific inhibitors of PI3 kinase and mTOR/FRAP.


Specificity / Sensitivity

Each phospho-specific antibody in the Translational Control Antibody Sampler Kit detects the intended target only when phosphorylated at the indicated site. Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites.


Source / Purification

Phospho-specific polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser209 of human elF4E. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Phospho-specific rabbit monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser473 of mouse Akt, Thr37 and Thr46 of mouse 4E-BP1, Ser51 of human eIF2α, Ser235 and Ser236 of human ribosomal protein S6, and Thr389 of human p70 S6 kinase.

Key steps in translational control occur at the level of eukaryotic initiation factor 4F (eIF4F) and p70 S6 kinase regulation. eIF4F is a complex whose functions include the recognition of the mRNA 5' cap structure. Several stimuli, such as insulin and various growth and survival factors, regulate the eIF4F complex and p70 S6 kinase primarily by triggering a signaling cascade dependent on sequential activation of PI3K, Akt/PKB and mTOR/FRAP kinases. Akt is activated by phosphorylation within the C-terminus at Ser473 and within the activation loop at Thr308 by phospholipid-dependent kinases. Inactivation in vivo of PI3K by the highly selective inhibitor LY294002 inhibits Akt and downstream elements of this cascade. Direct phosphorylation of mTOR/FRAP at Ser2448 by Akt is a key regulatory event controlling its kinase activity. mTOR/FRAP activity can be effectively blocked by Rapamycin, leading to inactivation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1), an inhibitor of translation initiation, and activation of p70 S6 kinases. Inactivation of 4E-BP1 by sequential phosphorylation causes the release of eIF4E, which, together with eIF4G and other factors, forms a functional eIF4F cap binding complex. p70 S6 kinases phosphorylates the 40S ribosomal subunit protein S6 and stimulates the translation of 5' oligopyrimidine tract containing mRNAs. The Erk pathway is also involved in regulation at this level by regulating the eIF4E kinase, Mnk1, and activating p70 S6 kinase. Tuberin, a product of the tumor supressor gene TSG2, is directly phosphorylated atThr1462 by Akt/PKB. Tuberin inhibits the mammalian target of rapamycin, mTOR, which results in inhibition of p70 S6 kinase and activation of 4E-BP1 and, therefore, inhibition of translation.


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6.  Dever, T.E. (2002) Cell 108, 545-556.

7.  Goncharova, E. et al. (2002) J. Biol. Chem. 277, 30958-30967.


Entrez-Gene Id 207, 208, 10000, 1965, 1977, 6198, 6194, 1978
Swiss-Prot Acc. P31749, P31751, Q9Y243, P05198, P06730, P23443, P62753, Q13541

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