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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-Syk (Tyr323) Antibody 2715 40 µl
Western Blotting Immunoprecipitation
H 72 Rabbit 
Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb 2710 40 µl
Western Blotting Immunoprecipitation Immunofluorescence Flow Cytometry
H 72 Rabbit IgG
Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody 2701 40 µl
Western Blotting Immunoprecipitation Immunofluorescence Flow Cytometry
H 70 Zap-70, 72 Syk Rabbit 
Syk (D3Z1E) XP® Rabbit mAb 13198 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Flow Cytometry
H M R 72 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 

Product Description

Phospho-Syk Sampler Kit provides an economical means to evaluate the activation status of Syk, including the phosphorylation of Tyr323, Tyr352 and Tyr525/526. The control Syk Antibody is also included. The kit contains enough primary and secondary antibodies for four Western blot experiments.


Specificity / Sensitivity

Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb detects endogenous levels of Syk protein only when phosphorylated at Tyr525/526 of human Syk or Tyr519/520 of mouse Syk. It also detects Syk protein when singly phosphorylated at Tyr526 of human Syk or Tyr520 of mouse Syk. Phospho-Syk (Tyr323) Antibody detects endogenous levels of Syk only when phosphorylated at Tyr323. Syk (D3Z1E) XP® Rabbit mAb recognizes endogenous levels of total Syk protein. All other antibodies in the kit do not cross-react with phosphorylated or nonphosphorylated forms of other related family members.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Tyr323 of human Syk or Tyr319 of human Zap-70. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues around Asn463 of human Syk protein or residues surrounding Tyr525/526 of human Syk.

Syk is a protein tyrosine kinase that plays an important role in intracellular signal transduction in hematopoietic cells (1-3). Syk interacts with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors (4). It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis (4). There is also evidence of a role for Syk in nonimmune cells and investigators have indicated that Syk is a potential tumor suppressor in human breast carcinomas (5). Tyr323 is a negative regulatory phosphorylation site within the SH2-kinase linker region in Syk. Phosphorylation at Tyr323 provides a direct binding site for the TKB domain of Cbl (6,7). Tyr352 of Syk is involved in the association of PLCγ1 (8). Tyr525 and Tyr526 are located in the activation loop of the Syk kinase domain; phosphorylation at Tyr525/526 of human Syk (equivalent to Tyr519/520 of mouse Syk) is essential for Syk function (9).


1.  Cheng, A.M. and Chan, A.C. (1997) Curr Opin Immunol 9, 528-33.

2.  Kurosaki, T. (1997) Curr Opin Immunol 9, 309-18.

3.  Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.

4.  Turner, M. et al. (2000) Immunol Today 21, 148-54.

5.  Coopman, P.J. et al. (2000) Nature 406, 742-7.

6.  Deckert, M. et al. (1998) J Biol Chem 273, 8867-74.

7.  Rao, N. et al. (2001) EMBO J 20, 7085-95.

8.  Law, C.L. et al. (1996) Mol Cell Biol 16, 1305-15.

9.  Zhang, J. et al. (2000) J Biol Chem 275, 35442-7.


Entrez-Gene Id 6850, 7535
Swiss-Prot Acc. P43405, P43403


For Research Use Only. Not For Use In Diagnostic Procedures.
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