Upstream / Downstream

Explore pathways related to this product.

Our U.S. Offices Are Closed

Our U.S. offices are closed in observance of Labor Day. We will reopen on Tuesday, September 2nd.

Thank you for your patience.


Find answers on our FAQs page.


Visit PhosphoSitePlus®

PTM information and tools available.


Product Includes Quantity Applications Reactivity MW(kDa) Isotype
p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb 4695 40 µl
H M R Hm Mk Mi Dm Z B Dg Pg Ce 42, 44 Rabbit 
SAPK/JNK (56G8) Rabbit mAb 9258 40 µl
H M R Hm Mk Mi 46, 54 Rabbit 
p38 MAPK (D13E1) XP® Rabbit mAb 8690 40 µl
H M R Hm Mk Pg 40 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
All Goat 

Product Description

The MAPK Family Antibody Sampler Kit provides an economical means of evaluating total levels of p38, p44/42, and SAPK/JNK mitogen-activated protein kinases. The kit contains enough primary and secondary antibody to perform four western blot experiments.

Specificity / Sensitivity

Each antibody in the MAPK Family Antibody Sampler Kit recognizes only its specific target. The antibodies do not cross-react with other MAP kinases.

Source / Purification

p44/42 MAP Kinase (137F5) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues near the C-terminus of rat p44 MAP kinase. SAPK/JNK (56G8) Rabbit mAb is produced by immunizing animals with a human JNK2/MBP fusion protein. p38 MAPK (D13E1) XP® Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human p38 protein.

p44/42 MAPK (Erk1/2), SAPK/JNK, and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, differentiation, and control of cellular responses to cytokines and stress. p44/42 MAPK is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine residues (Thr202 and Tyr204 in human Erk1) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). SAPK/JNK and p38 MAPK are activated by inflammatory cytokines and by a wide variety of cellular stresses. Activation of SAPK/JNK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* to activate p38 MAP kinase (1-5).

1.  Lewis, T. S. et al. (1998) Adv. Cancer Res. 74, 49-139.

2.  Garrington, T.P. and Johnson, G.L. (1999) Curr. Opin. Cell. Biol. 11, 211-218.

3.  Schaeffer, H.J. and Weber, M.J. (1999) Mol. Cell. Biol. 19, 2435-2444.

4.  Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485.

5.  Cobb, M.H. (1999) Prog. Biophys. Mol. Biol. 71, 479-500.

Entrez-Gene Id 5595, 5594, 1432, 5600, 6300, 5599
Swiss-Prot Acc. P27361, P28482, Q16539, Q15759, P53778, P45983

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.