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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
HDAC1 (10E2) Mouse mAb 5356 40 µl
Western Blotting Immunoprecipitation Immunofluorescence
H M R Mk 62 Mouse IgG1
HDAC2 (3F3) Mouse mAb 5113 40 µl
Western Blotting Immunoprecipitation Immunofluorescence
H M R Mk 60 Mouse IgG1
HDAC3 (7G6C5) Mouse mAb 3949 40 µl
Western Blotting Immunoprecipitation Immunofluorescence
H M R Mk 49 Mouse IgG2a
HDAC4 (D15C3) Rabbit mAb 7628 40 µl
Western Blotting Immunoprecipitation
H M R Mk 140 Rabbit IgG
Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5) Rabbit mAb 3443 40 µl
Western Blotting Immunoprecipitation
H M 140, 124, 120 Rabbit IgG
HDAC6 (D2E5) Rabbit mAb 7558 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence
H Mk 160 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
Western Blotting
All Horse 

Product Description

The Histone Deacetylase (HDAC) Antibody Sampler Kit provides a fast and economical means to evaluate the endogenous levels of HDACs. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.


Specificity / Sensitivity

The Histone Deacetylase (HDAC) Antibody Sampler Kit provides a fast and economical means to evaluate the endogenous levels of HDACs. The kit contains enough primary and secondary antibodies to perform four mini-blot experiments.

Each antibody in the Histone Deacetylase (HDAC) Antibody Sampler Kit detects endogenous levels of its target.


Source / Purification

Monoclonal antibodies are produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human HDAC6, the amino terminus of human HDAC4 protein, synthetic peptides to the carboxy-terminal residues of human HDAC1 and HDAC2 proteins, or synthetic phosphopeptide corresponding to Ser155 of human HDAC7 protein. HDAC3 (7G6C5) monoclonal antibody is produced by immunizing animals with recombinant human HDAC3 protein.

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).


1.  Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703.

2.  Gregory, P.D. et al. (2001) Exp Cell Res 265, 195-202.

3.  Liu, Y. et al. (2000) Mol Cell Biol 20, 5540-53.

4.  Cress, W.D. and Seto, E. (2000) J Cell Physiol 184, 1-16.

5.  Gray, S.G. and Ekström, T.J. (2001) Exp Cell Res 262, 75-83.

6.  Thiagalingam, S. et al. (2003) Ann. N.Y. Acad. Sci. 983, 84-100.

7.  Vigushin, D.M. and Coombes, R.C. (2004) Curr Cancer Drug Targets 4, 205-18.


Entrez-Gene Id 3065, 3066, 8841, 9759, 10014, 10013, 51564
Swiss-Prot Acc. Q13547, Q92769, O15379, P56524, Q9UQL6, Q9UBN7, Q8WUI4


For Research Use Only. Not For Use In Diagnostic Procedures.
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