Upstream / Downstream

Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
CDK2 (78B2) Rabbit mAb 2546 40 µl
Western Blotting Immunoprecipitation Flow Cytometry
H M R Mk 33 Rabbit 
p27 Kip1 (D69C12) XP® Rabbit mAb 3686 40 µl
Western Blotting Immunoprecipitation Immunofluorescence Flow Cytometry
H R Mk 27 Rabbit IgG
Cyclin D1 (92G2) Rabbit mAb 2978 40 µl
Western Blotting Immunohistochemistry Flow Cytometry
H M R 36 Rabbit IgG
CDK6 (DCS83) Mouse mAb 3136 40 µl
Western Blotting
H M R 36 Mouse IgG1
Cyclin D3 (DCS22) Mouse mAb 2936 40 µl
Western Blotting Immunohistochemistry
H M R 31 Mouse IgG1
p21 Waf1/Cip1 (12D1) Rabbit mAb 2947 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H Mk 21 Rabbit IgG
CDK4 (D9G3E) Rabbit mAb 12790 40 µl
Western Blotting Immunohistochemistry Immunofluorescence
H Mk 30 Rabbit IgG
p18 INK4C (DCS118) Mouse mAb 2896 40 µl
Western Blotting Immunoprecipitation
H 18 Mouse IgG2a
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
Western Blotting
All Horse 

Product Description

Cell Cycle Regulation Antibody Sampler kit offers an economical way of detecting eight integral cell cycle regulation proteins. The kit contains enough primary and secondary antibodies to perform four Western blot experiments with each primary antibody.


Specificity / Sensitivity

Antibodies detect endogenous levels of their respective proteins.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic peptides and are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with recombinant human proteins or synthetic peptides.

Eukaryotic cell cycle progression is dependent, in part, on the tightly regulated activity of cyclin dependent kinases (CDKs). Cyclin D/CDK4/6 activity occurs in mid-late G1 phase, upstream of CDK2/cyclin E activity. Both of these activities are required for hyperphosphorylation of the retinoblastoma gene product (pRb). pRb phosphorylation allows the release of S phase-promoting transcription factors and is indicative of the cell's commitment to proliferate. This point in the cell cycle is known as the restriction point. Cyclin protein levels oscillate throughout the cell cycle, and their availability is a means of controlling CDK activity and cell proliferation. Cyclin D is degraded through the ubiquitin proteasome pathway in the absence of mitogenic signaling. Ubiquitination of cyclin D1 is enhanced by phosphorylation at Thr286 by glycogen synthase kinase 3b (GSK-3b) (1). p27/Kip1, p57 Kip2 and p21 Waf1/Cip1 are members of the Cip/Kip family of cyclin-dependent kinase inhibitors. They form heterotrimeric complexes with cyclins and CDKs, inhibiting kinase activity and blocking progression through G1/S phase (2). However, p21 may enhance assembly and activity of cyclin D/CDK4/6 complexes (3). Levels of p21 and p27 protein are controlled through ubiquitination and proteasomal degradation (4). Levels of p27 are upregulated in quiescent cells and in cells treated with negative cell cycle regulators. p27 nuclear localization is controlled by Akt-dependent phosphorylation at Thr157 (5). The inhibitors of CDK4 (INK4) family include p15 INK4B, p16 INK4A, p18 INK4C, and p19 INK4D. All INK4 proteins selectively inhibit CDK4/6 activity, either in a binary complex, or in a ternary complex including cyclin D, resulting in inhibition of cell division (6,7).


1.  Diehl, J.A. et al. (1997) Genes Dev. 11, 957-972.

2.  Pestell, R.G. et al. (1999) Endocrine Rev. 20, 501-534.

3.  Cheng, J. et al. (1999) EMBO J. 18, 1571-1573.

4.  Sheaff, R.J. et al. (2000) Cell 5, 403-410.

5.  Shin, I. et al. (2002) Nat. Med. 8, 1145-1152.

6.  Guan, K.L. et al. (1994) Genes Dev. 8, 2939-2952.

7.  Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681.


Entrez-Gene Id 1017, 1019, 1021, 595, 896, 1031, 1026, 1027
Swiss-Prot Acc. P24941, P11802, Q00534, P24385, P30281, P42773, P38936, P46527


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.