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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Acetyl-Histone H2B (Lys5) (D5H1S) XP® Rabbit mAb 12799 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Chromatin Immunoprecipitation
H M R Mk 14 Rabbit IgG
Acetyl-Histone H2A (Lys5) Antibody 2576 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry
H M R Mk 14 Rabbit 
Histone H2A (D6O3A) Rabbit mAb 12349 40 µl
Western Blotting Immunofluorescence Chromatin Immunoprecipitation
H M R Mk Z 14 Rabbit IgG
Histone H2B (D2H6) Rabbit mAb 12364 40 µl
Western Blotting Immunohistochemistry Chromatin Immunoprecipitation
H M R Mk 14 Rabbit IgG
Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb 9649 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry Chromatin Immunoprecipitation
H M Z Ce 17 Rabbit IgG
Histone H3 (D1H2) XP® Rabbit mAb 4499 40 µl
Western Blotting Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Mk 17 Rabbit IgG
Acetyl-Histone H4 (Lys8) Antibody 2594 40 µl
Western Blotting
H M R Mk 11 Rabbit 
Histone H4 (L64C1) Mouse mAb 2935 40 µl
Western Blotting Immunohistochemistry
H M R Mk 11 Mouse IgG1
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 

Product Description

The Acetyl-Histone Antibody Sampler Kit provides a fast and economical means of evaluating the acetylation states of histones H2A, H2B, H3 and H4. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.


Specificity / Sensitivity

Each acetyl-histone antibody recognizes only the indicated protein target modified at the indicated site. Each control histone antibody recognizes the corresponding histone regardless of its acetylation state.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide and are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with recombinant human proteins or synthetic peptides.

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).


1.  Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.

2.  Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.

3.  Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.

4.  Cheung, P. et al. (2000) Cell 103, 263-71.

5.  Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.

6.  Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.

7.  Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.

8.  Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.

9.  Goto, H. et al. (1999) J Biol Chem 274, 25543-9.

10.  Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85.

11.  Dai, J. et al. (2005) Genes Dev 19, 472-88.


Entrez-Gene Id 8329 , 3018 , 8350 , 8359
Swiss-Prot Acc. P0C0S8 , P33778 , P68431 , P62805


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.