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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Jak1 (6G4) Rabbit mAb 3344 40 µl
Western Blotting Immunohistochemistry
H M R 130 Rabbit IgG
Jak2 (D2E12) XP® Rabbit mAb 3230 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R 125 Rabbit IgG
Jak3 (D1H3) Rabbit mAb 8827 40 µl
Western Blotting Immunoprecipitation Immunofluorescence
H 115 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 

Product Description

The Jak Isoform Antibody Sampler Kit provides an economical means to examine several Jak isoforms. The kit contains enough primary and secondary antibodies to perform four Western blot experiments per primary antibody.


Specificity / Sensitivity

Each Jak isoform antibody recognizes endogenous levels of the specified Jak isoform independent of its phosphorylation state. Neither antibody cross-reacts with the two other isoforms.


Source / Purification

Each isoform-specific antibody is produced by immunizing animals with a synthetic peptide that is specific for the Jak isoform. Jak1 and Jak3 antibodies are purified by protein A and peptide affinity chromatography. Each Jak antibody included in this kit is specific and does not cross-react with the other isoforms.

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.


1.  Darnell, J.E. (1997) Science 277, 1630-5.

2.  Leonard, W.J. (1998) Annu. Rev. Immunol. 16, 293-322.

3.  VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.

4.  Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.

5.  Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.


Entrez-Gene Id 3716 , 3717 , 3718
Swiss-Prot Acc. P23458 , O60674 , P52333


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select antibodies in this kit are sold under a license from Chemicon International, Inc. relating to U.S. Patent No. 5,658,791.
U.S. Patent No. 5,675,063.