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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-FoxO1 (Thr24)/FoxO3a (Thr32) Antibody 9464 40 µl
H M R Mk 78 to 82, 95 Rabbit 
Phospho-FoxO1 (Ser256) Antibody 9461 40 µl
H M R Mk 82 Rabbit 
Phospho-FoxO1 (Thr24)/FoxO3a (Thr32)/FoxO4 (Thr28) (4G6) Rabbit mAb 2599 40 µl
H M Mk 65, 78 to 82, 95 Rabbit 
FoxO1 (C29H4) Rabbit mAb 2880 40 µl
H M R Mk 78 to 82 Rabbit 
Phospho-FoxO3a (Ser253) (D18H8) Rabbit mAb 13129 40 µl
H M R Mk 97 Rabbit IgG
Phospho-FoxO3a (Ser318/321) Antibody 9465 40 µl
H M R Mk 97 Rabbit 
FoxO4 Antibody 9472 40 µl
H M R Mk 65 Rabbit 
FoxO3a (D19A7) Rabbit mAb 12829 40 µl
H M R 82 to 97 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
All Goat 

Product Description

This sampler kit provides an economical means to investigate Forkhead signaling. The kit contains primary and secondary antibodies to perform four Western blots with each antibody.


Specificity / Sensitivity

Phospho-FoxO1 (Thr24)/FoxO3a (Thr32) Antibody detects endogenous levels of FoxO1/FoxO3a only when phosphorylated at Thr24 of FoxO1 or Thr32 of FoxO3a. The antibody cross-reacts with phosphorylated FoxO4 at Thr28, but not with FoxO1 family members phosphorylated at other sites. Phospho-FoxO1 (Ser256) Antibody detects endogenous levels of FoxO1 only when phosphorylated at Ser256. The antibody cross-reacts with Fox04 phosphorylated at Ser193. Phospho-FoxO1 (Thr24)/FoxO3a (Thr32)/Fox04 (Thr28) (4G6) Rabbit mAb detects endogenous levels of FoxO1 when phosphorylated at Thr24, of FoxO3a when phosphorylated at Thr32 or FoxO4 when phosphorylated at Thr28. FoxO1 (C29H4) Rabbit mAb detects endogenous levels of total FoxO1 protein. The antibody does not detect exogenously expressed family members FoxO3a or FoxO4. Phospho-FoxO3a (Ser253) (D18H8) Rabbit mAb detects endogenous levels of FoxO3a only when phosphorylated at Ser253. This antibody may cross-react with FoxO1 when overexpressed and phosphorylated at Ser251 or FoxO4 when overexpressed and phosphorylated at Ser197. Phospho-FoxO3a (Ser318/321) Antibody detects endogenous levels of FoxO3a only when phosphorylated at Ser318/321. The antibody is expected to cross-react with FoxO1 when phosphorylated at Ser322/325 based on the peptide sequence. FoxO3a (D19A7) Rabbit mAb recognizes endogenous levels of total FoxO3a protein. The FoxO4 Antibody detects endogenous levels of FoxO4. The antibody is sensitive to phosphorylation within the antigen and preferrentially detects unphosphorylated FoxO4.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr28 of human Fox04, with a synthetic phosphopeptide corresponding to residues around Ser256 of human Fox01, or with a synthetic peptide corresponding to residues of human Fox04. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with recombinant protein specific to the carboxy terminus of human FoxO3 protein, with a GST-fusion protein corresponding to carboxy-terminal residues of human FoxO1, with a synthetic phosphopeptide corresponding to the sequence of human Fox03a, or with a synthetic phosphopeptide corresponding to residues surrounding Thr28 of human Fox04.

The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).


1.  Anderson, M.J. et al. (1998) Genomics 47, 187-99.

2.  Galili, N. et al. (1993) Nat Genet 5, 230-5.

3.  Borkhardt, A. et al. (1997) Oncogene 14, 195-202.

4.  Nakae, J. et al. (1999) J Biol Chem 274, 15982-5.

5.  Rena, G. et al. (1999) J Biol Chem 274, 17179-83.

6.  Guo, S. et al. (1999) J Biol Chem 274, 17184-92.

7.  Seoane, J. et al. (2004) Cell 117, 211-23.

8.  Arden, K.C. (2004) Mol Cell 14, 416-8.

9.  Yang, Y. et al. (2005) EMBO J 24, 1021-32.


Entrez-Gene Id 2308, 2309, 4303
Swiss-Prot Acc. Q12778, O43524, P98177


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.