Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Our U.S. Offices Are Closed

Our U.S. offices are closed in observance of Thanksgiving. We will reopen on Monday, December 1st.

Thank you for your patience.

To get local purchase information on this product, click here

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Toll-like Receptor 1 Antibody 2209 40 µl
Western Blotting Immunoprecipitation
H 86 Rabbit 
Toll-like Receptor 2 (D7G9Z) Rabbit mAb 12276 40 µl
Western Blotting Immunoprecipitation
H 90-105 Rabbit IgG
Toll-like Receptor 7 (D7) Rabbit mAb 5632 40 µl
Western Blotting Immunoprecipitation
H 140 Rabbit IgG
Toll-like Receptor 9 (D2C9) Rabbit mAb 5845 40 µl
Western Blotting Immunoprecipitation
H 130 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 

Product Description

The Toll-Like Receptor Antibody Sampler Kit is an economical way to examine the total protein levels of a number of toll-like receptors. This kit includes enough primary and secondary antibodies to perform four Western blot experiments with each antibody.


Specificity / Sensitivity

All antibodies contained in this kit detect endogenous levels of their respective total TLR protein. Cross reactivity was not detected with other family members at physiological conditions.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide and are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with recombinant human proteins or synthetic peptides.

Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm.


1.  Akira, S. (2003) J Biol Chem 278, 38105-8.

2.  Beutler, B. (2004) Nature 430, 257-63.

3.  Dunne, A. and O'Neill, L.A. (2003) Sci STKE 2003, re3.

4.  Medzhitov, R. et al. (1997) Nature 388, 394-7.

5.  Schwandner, R. et al. (1999) J Biol Chem 274, 17406-9.

6.  Takeuchi, O. et al. (1999) Immunity 11, 443-51.

7.  Alexopoulou, L. et al. (2001) Nature 413, 732-8.

8.  Zhang, F.X. et al. (1999) J Biol Chem 274, 7611-4.

9.  Horng, T. et al. (2001) Nat Immunol 2, 835-41.

10.  Oshiumi, H. et al. (2003) Nat Immunol 4, 161-7.

11.  Muzio, M. et al. (1997) Science 278, 1612-5.

12.  Wesche, H. et al. (1997) Immunity 7, 837-47.

13.  Suzuki, N. et al. (2002) Nature 416, 750-6.

14.  Irie, T. et al. (2000) FEBS Lett 467, 160-4.


Entrez-Gene Id 7096, 7097, 51284, 54106
Swiss-Prot Acc. Q15399, O60603, Q9NYK1, Q9NR96


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.