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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Toll-like Receptor 1 Antibody 2209 40 µl
Western Blotting Immunoprecipitation
H 86 Rabbit 
Toll-like Receptor 2 (D7G9Z) Rabbit mAb 12276 40 µl
Western Blotting Immunoprecipitation
H 90-105 Rabbit IgG
Toll-like Receptor 7 (D7) Rabbit mAb 5632 40 µl
Western Blotting Immunoprecipitation
H 140 Rabbit IgG
Toll-like Receptor 9 (D2C9) Rabbit mAb 5845 40 µl
Western Blotting Immunoprecipitation
H 130 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 

Product Description

The Toll-Like Receptor Antibody Sampler Kit is an economical way to examine the total protein levels of a number of toll-like receptors. This kit includes enough primary and secondary antibodies to perform four Western blot experiments with each antibody.


Specificity / Sensitivity

All antibodies contained in this kit detect endogenous levels of their respective total TLR protein. Cross reactivity was not detected with other family members at physiological conditions.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide and are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with recombinant human proteins or synthetic peptides.

Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm.


1.  Akira, S. (2003) J Biol Chem 278, 38105-8.

2.  Beutler, B. (2004) Nature 430, 257-63.

3.  Dunne, A. and O'Neill, L.A. (2003) Sci STKE 2003, re3.

4.  Medzhitov, R. et al. (1997) Nature 388, 394-7.

5.  Schwandner, R. et al. (1999) J Biol Chem 274, 17406-9.

6.  Takeuchi, O. et al. (1999) Immunity 11, 443-51.

7.  Alexopoulou, L. et al. (2001) Nature 413, 732-8.

8.  Zhang, F.X. et al. (1999) J Biol Chem 274, 7611-4.

9.  Horng, T. et al. (2001) Nat Immunol 2, 835-41.

10.  Oshiumi, H. et al. (2003) Nat Immunol 4, 161-7.

11.  Muzio, M. et al. (1997) Science 278, 1612-5.

12.  Wesche, H. et al. (1997) Immunity 7, 837-47.

13.  Suzuki, N. et al. (2002) Nature 416, 750-6.

14.  Irie, T. et al. (2000) FEBS Lett 467, 160-4.


Entrez-Gene Id 7096, 7097, 51284, 54106
Swiss-Prot Acc. Q15399, O60603, Q9NYK1, Q9NR96


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.